Human Integrin alpha V beta 3 PE-conjugated Antibody

Name: Human Integrin alpha V beta 3 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB3050P
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB3050P

Citations (4)

(1)

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Species Reactivity:	Human
Immunogen:	Human osteoclasts
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 23C6
Specificity:	Detects human Integrin alpha V beta 3.

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Applications for Human Integrin alpha V beta 3 PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Integrin alpha V beta 3 PE-conjugated Antibody

	Flow Cytometry Detection of Integrin alpha V beta 3 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Integrin aV beta 3 PE-conjugated Monoclonal Antibody (Catalog # FAB3050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Integrin alpha V beta 3

Integrin alpha V beta 3, together with Integrin alpha IIb beta 3, constitute the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg²⁺ or Mn²⁺ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow alpha V while the 685 aa human beta 3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta 3.

Item	Value
References	Hynes, R. O. (2002) Cell 110:673. Serini, G. et al. (2006) Exp. Cell Res. 312:651. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88. Xiong, J. et al. (2001) Science 294:339. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415. Djaffar, I. et al. (1994) Biochem. J. 300:67. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433. Faccio, R. et al. (2003) J. Clin. Invest. 111:749. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
Entrez Gene IDs	3685 (Human)
Alternate Names	CD51, CD51 antigen, Integrin alpha V beta 3, MSK8, VNRADKFZp686A08142, Vitronectin receptor subunit alpha, antigen identified by monoclonal L230, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

Citations for Human Integrin alpha V beta 3 PE-conjugated Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Integrin alpha V beta 3 PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Hedhli et al. (2018), Evaluation of a dimeric-cRGD peptide for targeted PET-CT imaging of peripheral angiogenesis in diabetic mice Sci Rep, 8(1):5401.
PMID: 29599497
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Hedhli et al. (2017), Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of ? V ? 3 Integrin Activity Sci Rep, 7(1):3185.
PMID: 28600529
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Shojaei F et al. (2012), Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer. J. Exp. Clin. Cancer Res., 31(0):26.
PMID: 22444159
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Chang MW et al. (2012), Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells. PLoS ONE, 7(5):e37935.
PMID: 22655084

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Human Integrin alpha V beta 3 PE-conjugated Antibody

Name: Adam Guess

Application: Flow

Sample Tested: Bone marrow cells

Species: Human

Verified Customer | Posted 08/01/2016

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Product Documents for Human Integrin alpha V beta 3 PE-conjugated Antibody

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Human Integrin alpha V beta 3 PE-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human Integrin alpha V beta 3 PE-conjugated Antibody

Scientific Data Examples for Human Integrin alpha V beta 3 PE-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: Integrin alpha V beta 3

Citations for Human Integrin alpha V beta 3 PE-conjugated Antibody (4)

Customer Reviews for Human Integrin alpha V beta 3 PE-conjugated Antibody (1)

Customer Images

Human Integrin alpha V beta 3 PE-conjugated Antibody

Product Documents for Human Integrin alpha V beta 3 PE-conjugated Antibody

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