HT1080 cells were stained with Mouse Anti-Human Integrin alphaV beta3 PE-conjugated Monoclonal Antibody (Catalog # FAB3050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Integrin alphaV beta3 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Integrin aV beta3 PE-conjugated Monoclonal Antibody (Catalog # FAB3050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alphaV beta3 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HT1080 human fibrosarcoma cell line and HUVEC human umbilical vein endothelial cells

Reviewed Applications

Read 1 review rated 4 using FAB3050P in the following applications:

Flow Cytometry (1 Review)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha V beta 3

Integrin alphaV beta3, together with Integrin alphaIIb beta3, constitute the only known beta3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alphaV/CD51 and 93 kDa beta3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alphaV beta3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alphaV beta3 is in the N-terminal head region, formed by interaction of the beta3 vWFA domain with the alphaV beta-propeller structure (4). The alphaV subunit contributes a thigh and a calf region, while the beta3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alphaV subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alphaV beta3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg²⁺ or Mn²⁺ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alphaV beta3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alphaV beta3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alphaV beta3 (4, 10). The 962 aa human alphaV ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow alphaV while the 685 aa human beta3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta3.

References

Hynes, R. O. (2002) Cell 110:673.
Serini, G. et al. (2006) Exp. Cell Res. 312:651.
Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
Xiong, J. et al. (2001) Science 294:339.
Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
Djaffar, I. et al. (1994) Biochem. J. 300:67.
McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.

Alternate Names

antigen identified by monoclonal L230, CD51, CD51 antigen, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), MSK8, Vitronectin receptor subunit alpha, VNRADKFZp686A08142

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

Additional Integrin alpha V beta 3 Products

Product Documents for Human Integrin alphaV beta3 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin alphaV beta3 PE-conjugated Antibody

For research use only

Citations
Reviews

Human Integrin alphaV beta3 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3050P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Integrin alpha V beta 3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alphaV beta3 PE-conjugated Antibody

Detection of Integrin alphaV beta3 in HT1080 cells by Flow Cytometry

Detection of Integrin alphaV beta3 in HUVEC Human Cells by Flow Cytometry.

Applications for Human Integrin alphaV beta3 PE-conjugated Antibody

Flow Cytometry

Reviewed Applications

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: Integrin alpha V beta 3

References

Alternate Names

Entrez Gene IDs

Gene Symbol

Additional Integrin alpha V beta 3 Products

Product Documents for Human Integrin alphaV beta3 PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human Integrin alphaV beta3 PE-conjugated Antibody