Human Integrin  alphaV beta3 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3050P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 23C6

View all Integrin alpha V beta 3 Antibodies »

Product Specifications

Immunogen

Human osteoclasts

Specificity

Detects human Integrin alphaV beta3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alphaV beta3 PE-conjugated Antibody

Detection of Integrin aV beta3 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of Integrin alphaV beta3 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Integrin aV beta3 PE-conjugated Monoclonal Antibody (Catalog # FAB3050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  alphaV beta3 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Reviewed Applications

Read 1 review rated 4 using FAB3050P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha V beta 3

Integrin alphaV beta3, together with Integrin  alphaIIb beta3, constitute the only known beta3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alphaV/CD51 and 93 kDa beta3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alphaV beta3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alphaV beta3 is in the N-terminal head region, formed by interaction of the beta3 vWFA domain with the alphaV beta-propeller structure (4). The alphaV subunit contributes a thigh and a calf region, while the beta3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alphaV subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alphaV beta3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alphaV beta3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alphaV beta3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alphaV beta3 (4, 10). The 962 aa human alphaV ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow  alphaV while the 685 aa human beta3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta3.

References

  1. Hynes, R. O. (2002) Cell 110:673.
  2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
  3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
  4. Xiong, J. et al. (2001) Science 294:339.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:67.
  8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
  9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
  11. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
  12. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.

Alternate Names

antigen identified by monoclonal L230, CD51, CD51 antigen, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), MSK8, Vitronectin receptor subunit alpha, VNRADKFZp686A08142

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

Additional Integrin alpha V beta 3 Products

Product Documents for Human Integrin  alphaV beta3 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Integrin  alphaV beta3 PE-conjugated Antibody

For research use only

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