Human Integrin  alpha6 beta1 Heterodimer Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7809

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2548B

View all Integrin alpha 6 beta 1 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived Human Integrin  alpha6 beta1 heterodimer
Phe24-Ser1012(Integrin alpha 6) and Gln21-Asp728 (Integrin beta1)
Accession # NP_000201

Specificity

Detects human Integrin  alpha6 beta1 in direct ELISAs. In direct ELISA, less than 1% of cross reactivity with recombinant human (rh) Integrin beta1 and recombinant mouse (rm) Integrin alpha6 is observed. In direct ELISA, no cross-reactivity with rhIntegrin alpha3, beta2, beta3, beta5, beta6, beta7, and rmIntegrin beta1 is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Integrin  alpha6 beta1 Heterodimer Antibody

Detection of Integrin a6 beta1 antibody in Human PBMC antibody by Flow Cytometry

Detection of Integrin alpha6 beta1 in Human PBMC by Flow Cytometry

Human PBMC were stained with (A) Rabbit Anti-Human Integrin a6 beta1 Monoclonal Antibody (Catalog # MAB7809) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  alpha6 beta1 Heterodimer Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 6 beta 1

Integrin alpha6 beta1, also called platelet glycoprotein GPIc-IIa, is a laminin binding integrin that is expressed on T cells, monocytes, endothelial cells, stem cells, and platelets (1-9). The non-covalent heterodimer is composed of ~150 kDa alpha6/CD49f and 130 kDa beta1/CD29 type I transmembrane glycoprotein subunits (2). While alpha6 pairs only with beta1 or beta4, twelve integrins share the beta1 subunit (1-5). The alpha6 subunit is cleaved into extracellular heavy and transmembrane light chains (3). Alternative splicing in the human alpha6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a mouse or human cytoplasmic site creates A and B isoforms (10, 11). These forms do not appear to alter the binding specificity (4, 10, 11). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The human alpha6 (X1) heavy chain shares 94‑95% aa identity with mouse, rat, bovine, and canine alpha6, and the human beta1 ECD shares 92‑96% aa sequence identity with rat, bovine, mouse, and feline beta1. alpha6 beta1 shows broad specificity for adhesion to laminin isoforms (4, 10). Its expression on human and mouse pluripotent stem cells is important for attachment, expansion, and self‑renewal on LN‑511 (laminin  alpha5  beta1 gamma1) (6, 7). The secreted protein Netrin-4 and the laminin  gamma1 subunit form an adhesion‑activating complex with alpha6 beta1 on mouse neural stem cells and human lymphatic endothelial cells that promotes lymphangiogenesis (8, 9). alpha6 beta1 up‑regulation on cancers such as prostate, glioma, and hepatoma is reported to enhance tumorigenicity, motility, invasion and metastasis (12‑14). alpha6 beta1 cleavage via uPA (urokinase-type plasminogen activator) facilitates tumorigenicity in prostate cancers, and interaction of hepatoma alpha6 beta1 with EMMPRIN/CD147 may also enhance tumorigenicity by inducing uPA and other metalloproteinases (12, 13).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Tamura, R.N. et al. (1990) J. Cell Biol. 111:1593.
  4. Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
  5. Sonnenberg, A. and C.J.T. Linders (1990) J. Cell Science 96:207.
  6. Rodin, S. et al. (2010) Nat. Biotech. 28:611.
  7. Domogatskaya A. et al. (2008) Stem Cells 26:2800.
  8. Staquicini, F.I. et al. (2009) Proc. Natl. Acad. Sci. USA 106:2903.
  9. Larrieu-Lahargue, F. et al. (2011) Circ. Res. 109:770.
  10. Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
  11. Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
  12. Sroka, I.C. et al. (2011) Mol. Cancer Res. 9:1319.
  13. Dai, J.Y. et al. (2009) BMC Cancer 9:337.
  14. Delamarre, E. et al. (2009) Am. J. Pathol. 175:844.

Alternate Names

CD49f, ITGA6B, VLA-6

UniProt

NP_000201

Additional Integrin alpha 6 beta 1 Products

Product Documents for Human Integrin  alpha6 beta1 Heterodimer Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Integrin  alpha6 beta1 Heterodimer Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.