Human Integrin  alpha6 beta1 Heterodimer Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7809N

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2548B

View all Integrin alpha 6 beta 1 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived Human Integrin  alpha6 beta1 heterodimer
Phe24-Ser1012(Integrin alpha 6) and Gln21-Asp728 (Integrin beta1)
Accession # NP_000201

Specificity

Detects human Integrin  alpha6 beta1 in direct ELISAs. In direct ELISA, less than 1% of cross reactivity with recombinant human (rh) Integrin beta1 and recombinant mouse (rm) Integrin alpha6 is observed. In direct ELISA, no cross-reactivity with rhIntegrin alpha3, beta2, beta3, beta5, beta6, beta7, and rmIntegrin beta1 is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human PBMC

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 6 beta 1

Integrin alpha6 beta1, also called platelet glycoprotein GPIc-IIa, is a laminin binding integrin that is expressed on T cells, monocytes, endothelial cells, stem cells, and platelets (1-9). The non-covalent heterodimer is composed of ~150 kDa alpha6/CD49f and 130 kDa beta1/CD29 type I transmembrane glycoprotein subunits (2). While alpha6 pairs only with beta1 or beta4, twelve integrins share the beta1 subunit (1-5). The alpha6 subunit is cleaved into extracellular heavy and transmembrane light chains (3). Alternative splicing in the human alpha6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a mouse or human cytoplasmic site creates A and B isoforms (10, 11). These forms do not appear to alter the binding specificity (4, 10, 11). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The human alpha6 (X1) heavy chain shares 94‑95% aa identity with mouse, rat, bovine, and canine alpha6, and the human beta1 ECD shares 92‑96% aa sequence identity with rat, bovine, mouse, and feline beta1. alpha6 beta1 shows broad specificity for adhesion to laminin isoforms (4, 10). Its expression on human and mouse pluripotent stem cells is important for attachment, expansion, and self‑renewal on LN‑511 (laminin  alpha5  beta1 gamma1) (6, 7). The secreted protein Netrin-4 and the laminin  gamma1 subunit form an adhesion‑activating complex with alpha6 beta1 on mouse neural stem cells and human lymphatic endothelial cells that promotes lymphangiogenesis (8, 9). alpha6 beta1 up‑regulation on cancers such as prostate, glioma, and hepatoma is reported to enhance tumorigenicity, motility, invasion and metastasis (12‑14). alpha6 beta1 cleavage via uPA (urokinase-type plasminogen activator) facilitates tumorigenicity in prostate cancers, and interaction of hepatoma alpha6 beta1 with EMMPRIN/CD147 may also enhance tumorigenicity by inducing uPA and other metalloproteinases (12, 13).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Tamura, R.N. et al. (1990) J. Cell Biol. 111:1593.
  4. Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
  5. Sonnenberg, A. and C.J.T. Linders (1990) J. Cell Science 96:207.
  6. Rodin, S. et al. (2010) Nat. Biotech. 28:611.
  7. Domogatskaya A. et al. (2008) Stem Cells 26:2800.
  8. Staquicini, F.I. et al. (2009) Proc. Natl. Acad. Sci. USA 106:2903.
  9. Larrieu-Lahargue, F. et al. (2011) Circ. Res. 109:770.
  10. Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
  11. Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
  12. Sroka, I.C. et al. (2011) Mol. Cancer Res. 9:1319.
  13. Dai, J.Y. et al. (2009) BMC Cancer 9:337.
  14. Delamarre, E. et al. (2009) Am. J. Pathol. 175:844.

Alternate Names

CD49f, ITGA6B, VLA-6

UniProt

NP_000201

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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