Detects human Integrin alpha6 beta1 in direct ELISAs. In direct ELISA, less than 1% of cross reactivity with recombinant human (rh) Integrin beta1 and recombinant mouse (rm) Integrin alpha6 is observed. In direct ELISA, no cross-reactivity with rhIntegrin alpha3, beta2, beta3, beta5, beta6, beta7, and rmIntegrin beta1 is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications

Application

Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Integrin alpha 6 beta 1

Integrin alpha6 beta1, also called platelet glycoprotein GPIc-IIa, is a laminin binding integrin that is expressed on T cells, monocytes, endothelial cells, stem cells, and platelets (1-9). The non-covalent heterodimer is composed of ~150 kDa alpha6/CD49f and 130 kDa beta1/CD29 type I transmembrane glycoprotein subunits (2). While alpha6 pairs only with beta1 or beta4, twelve integrins share the beta1 subunit (1-5). The alpha6 subunit is cleaved into extracellular heavy and transmembrane light chains (3). Alternative splicing in the human alpha6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a mouse or human cytoplasmic site creates A and B isoforms (10, 11). These forms do not appear to alter the binding specificity (4, 10, 11). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The human alpha6 (X1) heavy chain shares 94‑95% aa identity with mouse, rat, bovine, and canine alpha6, and the human beta1 ECD shares 92‑96% aa sequence identity with rat, bovine, mouse, and feline beta1. alpha6 beta1 shows broad specificity for adhesion to laminin isoforms (4, 10). Its expression on human and mouse pluripotent stem cells is important for attachment, expansion, and self‑renewal on LN‑511 (laminin alpha₅ beta₁ gamma₁) (6, 7). The secreted protein Netrin-4 and the laminin gamma₁ subunit form an adhesion‑activating complex with alpha6 beta1 on mouse neural stem cells and human lymphatic endothelial cells that promotes lymphangiogenesis (8, 9). alpha6 beta1 up‑regulation on cancers such as prostate, glioma, and hepatoma is reported to enhance tumorigenicity, motility, invasion and metastasis (12‑14). alpha6 beta1 cleavage via uPA (urokinase-type plasminogen activator) facilitates tumorigenicity in prostate cancers, and interaction of hepatoma alpha6 beta1 with EMMPRIN/CD147 may also enhance tumorigenicity by inducing uPA and other metalloproteinases (12, 13).

References

Takada, Y. et al. (2007) Genome Biol. 8:215.
Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
Tamura, R.N. et al. (1990) J. Cell Biol. 111:1593.
Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
Sonnenberg, A. and C.J.T. Linders (1990) J. Cell Science 96:207.
Rodin, S. et al. (2010) Nat. Biotech. 28:611.
Domogatskaya A. et al. (2008) Stem Cells 26:2800.
Staquicini, F.I. et al. (2009) Proc. Natl. Acad. Sci. USA 106:2903.
Larrieu-Lahargue, F. et al. (2011) Circ. Res. 109:770.
Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
Sroka, I.C. et al. (2011) Mol. Cancer Res. 9:1319.
Dai, J.Y. et al. (2009) BMC Cancer 9:337.
Delamarre, E. et al. (2009) Am. J. Pathol. 175:844.

Alternate Names

CD49f, ITGA6B, VLA-6

UniProt

NP_000201

Additional Integrin alpha 6 beta 1 Products

Product Documents

Product Datasheets

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COA

SDS

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Product Specific Notices

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Human Integrin alpha6 beta1 Heterodimer Alexa Fluor™ Plus 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7809AFP405

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Integrin alpha 6 beta 1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications

CyTOF-ready

Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Shipping

Stability & Storage

Background: Integrin alpha 6 beta 1

References

Alternate Names

UniProt

Additional Integrin alpha 6 beta 1 Products

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices

CONTACT INFORMATION

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