Western blot shows lysates of human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL-2 (Catalog # 202-IL), Recombinant Human IL-4 (Catalog # 204-IL), and Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL-2 (Catalog # 202-IL), Recombinant Human IL-4 (Catalog # 204-IL), Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF-beta 1 (Catalog # 240-B). PVDF membrane was probed with a 0.1 µg/mL of Rabbit Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human IL-9 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), and Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF‑ beta1 (Catalog # 240-B)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2091 in the following applications:

ELISA (1 Review)
Immunoprecipitation (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9

Interleukin-9 (IL-9) is a heavily glycosylated cytokine with a 14 kDa peptide backbone that is secreted by CD4⁺ Th2 cells. It supports the growth of multiple hematopoietic cell types including Th cells, germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.

Long Name

Interleukin 9

Alternate Names

IL9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Gene Symbol

IL9

UniProt

P15248

Additional IL-9 Products

Product Documents for Human IL-9 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-9 Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Citations
Reviews

Human IL-9 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2091

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all IL-9 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-9 Antibody

Detection of Human IL‑9 by Western Blot.