Detects human IL-13 R alpha1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-13 R alpha1, recombinant human (rh) IL-13 R alpha2, rhIL-4 R, rhIL-5 R beta, or rhIL-9 R is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human IL-13 R alpha1 Antibody

Detection of Human IL-13 R alpha1 by Western Blot.

Western blot shows lysates of human heart tissue and human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-13 Ra1 Monoclonal Antibody (Catalog # MAB1462) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-13 Ra1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of IL‑13 R alpha1 in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and (B) Mouse Anti-Human IL-13 Ra1 Monoclonal Antibody (Catalog # MAB1462, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Applications for Human IL-13 R alpha1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood granulocytes

Western Blot

2 µg/mL
Sample: Human heart tissue and human skeletal muscle tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13 R alpha 1

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha or NR4, is now referred to as IL-13 R alpha1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha', is now referred to as IL-13 R alpha2.

The human IL-13 R alpha1 was originally cloned based on sequence homology to the mouse IL-13 R alpha1. The IL-13 R alpha1 cDNA encodes a 427 amino acid (aa) precursor protein with a putative 21 aa signal peptide, a 324 aa extracellular domain, a 23 aa transmembrane region and a 59 aa cytoplasmic tail. Human and mouse IL-13 R alpha1 share 76% aa sequence identity. The extracellular domain of IL-13 R alpha1 is also closely related to that of IL-13 R alpha2. IL‑13 R alpha1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha2 in IL-13 signaling remains to be elucidated.

References

Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.

Long Name

Interleukin 13 Receptor alpha 1

Alternate Names

CD213a1, IL-13Ra1, IL13R alpha 1, IL13RA1

Entrez Gene IDs

3597 (Human); 16164 (Mouse)

Gene Symbol

IL13RA1

UniProt

P78552

Additional IL-13 R alpha 1 Products

Product Documents for Human IL-13 R alpha1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-13 R alpha1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IL-13 R alpha1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1462

Key Product Details

Species Reactivity

Validated:

Cited:

Applications