Human IL-13 Antibody

Name: Human IL-13 Antibody
Brand: R&D Systems
SKU: MAB2131
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB2131

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL-13 Gly35-Asn146 Accession # P35225
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 31606
Specificity:	Detects human IL-13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL‑4 or recombinant mouse IL-13 is observed.

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Applications for Human IL-13 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑13 (Catalog # 213-ILB)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	0.25 µg/10⁶ cells	See below

Item	Value
Neutralization	Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑13.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-13 Antibody

	Intracellular Staining by Flow Cytometry Detection of IL‑13 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 5 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB2131) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
	Neutralization Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 Antibody. Recombinant Human IL-13 (Catalog # 213-ILB) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB2131). The ND₅₀ is typically 1-4 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

Item	Value
References	Wills-Karp, M. (2004) Immunol. Rev. 202:175. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231. Ruetten, H. and C. Thiemermann (1997) Shock 8:409. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464. Tabata, Y. et al. (2007) J. Immunol. 177:7905. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
Long Name	Interleukin 13
Entrez Gene IDs	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Alternate Names	ALRHMGC116789, BHR1interleukin-13, IL-13, IL-13MGC116788, IL13, MGC116786, NC30, P600, interleukin 13

Citations for Human IL-13 Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-13 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Blocking

van Meijgaarden K et al. (2015), Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset. PLoS Pathog, 11(3):e1004671.
PMID: 25803478
Species: Rat
Sample Types: Tissue Homogenates
Applications: Neutralization

Haas CS et al. (2007), In vivo inhibition of angiogenesis by interleukin-13 gene therapy in a rat model of rheumatoid arthritis. Arthritis Rheum., 56(8):2535-48.
PMID: 17665443

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Customer Reviews for Human IL-13 Antibody (2)

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Human IL-13 Antibody

Name: Anonymous

Application: Luminex

Sample Tested: Serum

Species: Human

Verified Customer | Posted 11/08/2018
Human IL-13 Antibody

Name: Balaji Mahender

Application: ELISA

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 01/18/2018

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Product Documents for Human IL-13 Antibody

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Human IL-13 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human IL-13 Antibody

Scientific Data Examples for Human IL-13 Antibody

Intracellular Staining by Flow Cytometry

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-13

Citations for Human IL-13 Antibody (2)

Customer Reviews for Human IL-13 Antibody (2)

Human IL-13 Antibody

Human IL-13 Antibody

Product Documents for Human IL-13 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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