Human IFN-gamma Antibody

Name: Human IFN-gamma Antibody
Brand: R&D Systems
SKU: AF-285-NA
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF-285-NA

Citations (13)

(3)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IFN-gamma Met1-Gln144 Accession # Q14609
Applications:	Block/Neutralize, Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human IFN-gamma in direct ELISAs and Western blots.

View Full Technical Details for Human IFN-gamma Antibody

Applications for Human IFN-gamma Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IFN-gamma (Catalog # 285-IF)
Immunocytochemistry	5-15 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize IFN-gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.0300 - 0.300 µg/mL in the presence of 5.00 ng/mL Recombinant Human IFN-gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IFN-gamma Antibody

	Neutralization IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN-gamma (285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA). The ND₅₀ is typically 0.0300 ‑ 0.300 µg/mL.
	Immunocytochemistry IFN‑ gamma in Human PBMCs. IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red); (NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Item	Value
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Human IFN-gamma Antibody (13)

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Citations are publications that use Bio-Techne products. Selected citations for Human IFN-gamma Antibody include:

Species: Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC

H Zheng et al. (2022), Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques Cell Reports, 39(8):110864.
PMID: 35594870
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

L Shan et al. (2020), Killer cell proteases can target viral immediate-early proteins to control human cytomegalovirus infection in a noncytotoxic manner PLoS Pathog., 16(4):e1008426.
PMID: 32282833
Species: Xenograft
Sample Types: Whole Tissue
Applications: CAR-T (IHC-Fr)

F Zou et al. (2019), Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56 Nat Commun, 10(1):4109.
PMID: 31511513
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Zheng J et al. (2015), The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice. PLoS ONE, 10(8):e0135999.
PMID: 26285203
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Mishto M et al. (2011), Immunoproteasome expression is induced in mesial temporal lobe epilepsy. Biochem. Biophys. Res. Commun., 408(1):65-70.
PMID: 21458417
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Knoblach T et al. (2011), Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma. PLoS Pathog., 7(4):e1002016.
PMID: 21533215
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Heo YJ et al. (2010), IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Immunol. Lett., 127(2):150-6.
PMID: 19895848
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Guo PF et al. (2010), Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Blood, 116(12):2061-9.
PMID: 20538796
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Kobayashi A et al. (2008), Evolving immunosuppressive microenvironment during human cervical carcinogenesis. Mucosal Immunol, 1(5):412-20.
PMID: 19079205
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Liu TY et al. (2008), Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 38(4):1012-23.
PMID: 18350544
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Chi W et al. (2008), Upregulated IL-23 and IL-17 in Behcet patients with active uveitis. Invest. Ophthalmol. Vis. Sci., 49(7):3058-64.
PMID: 18579762
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Hessian PA et al. (2003), Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma. Arthritis Rheum., 48(2):334-8.
PMID: 12571841
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Wang R et al. (0), Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1. J Leukoc Bio91(2):299-309.
PMID: 22045868

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Customer Reviews for Human IFN-gamma Antibody (3)

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Human IFN-gamma Antibody

Name: Stephanie Zandee

Application: IHC

Sample Tested: Adult brain

Species: Human

Verified Customer | Posted 04/13/2018

Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287Used at 10ug/ml.Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).
Human IFN-gamma Antibody

Name: Becker Law

Application: ICC/IF

Sample Tested: Kidney tissue (cortex)

Species: Human

Verified Customer | Posted 10/01/2017
Human IFN-gamma Antibody

Name: Shijun Yan

Application: ELISA

Sample Tested: activated mouse CD8 T cell

Species: Mouse

Verified Customer | Posted 06/29/2016

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Human IFN-gamma Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IFN-gamma Antibody

Scientific Data Examples for Human IFN-gamma Antibody

Neutralization

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IFN-gamma

Citations for Human IFN-gamma Antibody (13)

Customer Reviews for Human IFN-gamma Antibody (3)

Customer Images

Human IFN-gamma Antibody

Human IFN-gamma Antibody

Human IFN-gamma Antibody

Product Documents for Human IFN-gamma Antibody

Product Datasheets

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