Has the human gp130 monoclonal antibody (Clone 28126; Catalog # MAB228) been tested in Western blot?

Name: Human gp130 Antibody
Brand: R&D Systems
SKU: MAB228

Our scientists have found that this antibody does not detect linearized, denatured protein.

Human gp130 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB228

Citations (4)

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 28126
Specificity:	Detects human gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R, rhIL-4 R, or rhIL-6 R is observed.

View Full Technical Details for Human gp130 Antibody

Applications for Human gp130 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Human whole blood monocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Item	Value
Neutralization	Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.04‑0.2 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human gp130 Antibody

	Neutralization Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF-1 human erythroleukemic cell line (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228). The ND₅₀ is typically 0.04-0.2 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: gp130

Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).

Item	Value
References	Wang, Y. et al. (1992) Genomics 14:666. Muller-Newen, G. (2003) Sci. STKE pe40. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
Long Name	Glycoprotein 130
Entrez Gene IDs	3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names	CD130, CD130 antigen, CDw130, DKFZp564F053, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, IL6ST, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form, interleukin 6 signal transducer (gp130, oncostatin M receptor), interleukin receptor beta chain, interleukin-6 receptor subunit beta, membrane glycoprotein gp130

Citations for Human gp130 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human gp130 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

AN Wilkinson et al. (2018), Granulocytes Are Unresponsive to IL-6 Due to an Absence of gp130 J. Immunol., 0(0).
PMID: 29626088
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ara T et al. (2009), Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. Cancer Res., 69(1):329-37.
PMID: 19118018
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Erices A et al. (2002), Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells. Exp. Cell Res., 280(1):24-32.
PMID: 12372336
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ambarus C et al. (0), Systematic validation of specific phenotypic markers for in vitro polarized human macrophages. J Immunol Method375(1):196-206.
PMID: 22075274

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FAQs for Human gp130 Antibody

Has the human gp130 monoclonal antibody (Clone 28126; Catalog # MAB228) been tested in Western blot?

Our scientists have found that this antibody does not detect linearized, denatured protein.

Human gp130 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human gp130 Antibody

Scientific Data Examples for Human gp130 Antibody

Neutralization

Formulation, Preparation and Storage

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Background: gp130

Citations for Human gp130 Antibody (4)

FAQs for Human gp130 Antibody

Product Documents for Human gp130 Antibody

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