Human gp130 APC-conjugated Antibody

Name: Human gp130 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB228A

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB228A

Citations (2)

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Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 28126
Specificity:	Detects human gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R, rhIL-4 R, or rhIL-6 R is observed.

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Applications for Human gp130 APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human gp130 APC-conjugated Antibody

	Flow Cytometry Detection of gp130 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human gp130 APC-conjugated Monoclonal Antibody (Catalog # FAB228A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: gp130

Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2-5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).

Item	Value
References	Wang, Y. et al. (1992) Genomics 14:666. Muller-Newen, G. (2003) Sci. STKE pe40. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
Long Name	Glycoprotein 130
Entrez Gene IDs	3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names	CD130, CD130 antigen, CDw130, DKFZp564F053, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, IL6ST, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form, interleukin 6 signal transducer (gp130, oncostatin M receptor), interleukin receptor beta chain, interleukin-6 receptor subunit beta, membrane glycoprotein gp130

Citations for Human gp130 APC-conjugated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human gp130 APC-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kolodziej S et al. (2014), PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation. Nat Commun, 5(0):3995.
PMID: 24874575
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Madrid et al. (2012), Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44. J Virol, 86(16):8693-704.
PMID: 22674989

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Human gp130 APC-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human gp130 APC-conjugated Antibody

Scientific Data Examples for Human gp130 APC-conjugated Antibody

Flow Cytometry