Human gp130 Antibody

Name: Human gp130 Antibody
Brand: R&D Systems
SKU: MAB2281

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB2281

Citations (4)

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain Leu24-Glu619 (Glu619Asp) Accession # P40189
Applications:	ELISA
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 29104
Specificity:	Detects human gp130 in direct ELISAs. In direct ELISAs no cross‑reactivity with recombinant mouse gp130 is observed.

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Applications for Human gp130 Antibody

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human gp130 Antibody

	ELISA Human gp130 ELISA Standard Curve. Recombinant Human gp130 protein was serially diluted 2-fold and captured by Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB2281) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: gp130

Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).

Item	Value
References	Wang, Y. et al. (1992) Genomics 14:666. Muller-Newen, G. (2003) Sci. STKE pe40. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
Long Name	Glycoprotein 130
Entrez Gene IDs	3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names	CD130, CD130 antigen, CDw130, DKFZp564F053, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, IL6ST, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form, interleukin 6 signal transducer (gp130, oncostatin M receptor), interleukin receptor beta chain, interleukin-6 receptor subunit beta, membrane glycoprotein gp130

Citations for Human gp130 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human gp130 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

H Wang et al. (2021), Targeting EphA2 suppresses hepatocellular carcinoma initiation and progression by dual inhibition of JAK1/STAT3 and AKT signaling Cell Reports, 34(8):108765.
PMID: 33626345
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Y Hosokawa et al. (2017), IL-27 Modulates Chemokine Production in TNF-? -Stimulated Human Oral Epithelial Cells Cell. Physiol. Biochem., 43(3):1198-1206.
PMID: 28977795
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Campbell I et al. (2014), Trans-signaling is a dominant mechanism for the pathogenic actions of interleukin-6 in the brain. J Neurosci, 34(7):2503-13.
PMID: 24523541
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Ammit AJ et al. (2007), Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle. Am. J. Physiol. Lung Cell Mol. Physiol., 292(1):L199-206.
PMID: 16936245

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