Human Galectin-3 Antibody

Name: Human Galectin-3 Antibody
Brand: R&D Systems
SKU: MAB11542
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB11542

(1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human Galectin-3 Ala2-Ile250 Accession # P17931
Applications:	CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 194801
Specificity:	Detects human Galectin-3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Galectin-3 is observed and no cross-reactivity with recombinant human (rh) Galectin-8 or rhGalectin-9 is observed.

View Full Technical Details for Human Galectin-3 Antibody

Applications for Human Galectin-3 Antibody

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Galectin-3 Antibody

	Western Blot Detection of Human Galectin‑3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin‑3 Monoclonal Antibody (Catalog # MAB11542) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin‑3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Intracellular Staining by Flow Cytometry Detection of Galectin-3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11542, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Sterile PBS to a final concentration of 0.5 mg/mL. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35 and epsilon BP, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts.

Item	Value
Entrez Gene IDs	3958 (Human); 16854 (Mouse)
Alternate Names	AGE-R3, CBP 35,35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBPCBP35, GALIG, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin-3, Galectin3, IgE-binding protein, L-31, L29, L31, LGALS2, LGALS3, Laminin-binding protein, Lectin L-29, MAC2GAL3, Mac-2, Mac-2 antigen, galactin-3, lectin, galactoside-binding, soluble, 3

Citations for Human Galectin-3 Antibody

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Human Galectin-3 Antibody (1)

4 out of 5

1 customer rating

5 stars

4 stars

100%

3 stars

2 stars

1 star

Showing 1 - 1 of 1 review Showing All

Filter By:

Human Galectin‑3 Antibody

Name: Anonymous

Application: B/N

Sample Tested: Human recombinant antibody

Species: Goat

Verified Customer | Posted 04/26/2016

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

Product Documents for Human Galectin-3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human Galectin-3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Human Galectin-3 Antibody

Scientific Data Examples for Human Galectin-3 Antibody

Western Blot

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Galectin-3

Citations for Human Galectin-3 Antibody

Customer Reviews for Human Galectin-3 Antibody (1)

Human Galectin‑3 Antibody

Product Documents for Human Galectin-3 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected