Human Galectin‑3 PE‑conjugated Antibody

Catalog # IC11542P | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 194801

Product Summary for Human Galectin‑3 PE‑conjugated Antibody

Immunogen

E. coli-derived recombinant human Galectin-3
Ala2-Ile250
Accession # P17931

Specificity

Detects human Galectin-3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Galectin-3 is observed and no cross-reactivity with recombinant human (rh) Galectin-8 or rhGalectin-9 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Galectin‑3 PE‑conjugated Antibody

Detection of Galectin-3 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of Galectin‑3 in Human Blood Monocytes by Flow Cytometry.

LPS-treated peripheral blood monocytes were stained with Mouse Anti-Human Galectin-3 PE-conjugated Monoclonal Antibody (Catalog # IC11542P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Galectin‑3 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: LPS-treated peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35 and IgE Binding Protein, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their Carbohydrate Recognition Domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts where it forms homodimers, or heterodimers, likely with DMBT1. It is reported to complex with Integrins alpha3 and beta1. Galectin-3 is known to circulate and show elevation in inflammatory disorders.

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Entrez Gene IDs

3958 (Human); 16854 (Mouse)

Gene Symbol

LGALS3

UniProt

P17931

Product Documents for Human Galectin‑3 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Galectin‑3 PE‑conjugated Antibody

For research use only

Setting the standard in quality research reagents