Human FAK Antibody

Detection of Human FAK by Simple Western^TM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FAK at approximately 120 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human FAK Monoclonal Antibody (Catalog # MAB4467) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human FAK by Western Blot

Phosphorylation of FAK following treatment of AF cells derived from non-degenerate IVDs with 1.0 Hz CTS, with and without pre-treatment with RGD or RAD peptides.AF cells derived from non-degenerate IVDs (n = 4) were treated+/− RGD (50 µg/ml) or RAD (50 µg/ml) peptides, and mechanically stimulated (10% CTS, 1.0 Hz frequency) in serum-free media and total protein extracted at timepoints of 5 and 20 minutes. Mechanically stimulated and unstimulated,+/− RGD or RAD peptides, non-degenerate protein samples (5 µg/well) exposed to A) 5 minutes and B) 20 minutes of CTS, were separated using 10% SDS-PAGE and probed using primary antibodies against phosphorylated FAK. Blots were then stripped using a stripping buffer, re-blocked and probed using an antibody against total FAK protein. C) The density of bands were quantified using a Syngene imaging system and the ratio of phosphorylated: total FAK protein normalised to timepoint controls and plotted as % change. *denotes a significant change (p≤0.05) between treatment groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24039840), licensed under a CC-BY license. Not internally tested by R&D Systems.