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Human FAK Antibody

Catalog # MAB4467 | R&D Systems, Inc. a Bio-Techne Brand
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MAB4467
MAB4467-SP

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 495919

Product Summary for Human FAK Antibody

Immunogen

E. coli-derived recombinant human FAK
Asp213-Thr412
Accession # Q05397

Specificity

Detects human FAK in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FAK Antibody

Detection of Human FAK antibody by Western Blot.

Detection of Human FAK by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, A549 human lung carcinoma cell line, Huh-7 human hepatoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
FAK antibody in Human Brain by Immunohistochemistry (IHC-P).

FAK in Human Brain.

FAK was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human FAK Monoclonal Antibody (Catalog # MAB4467) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot Shows Human FAK Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human FAK Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and FAK knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FAK Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Knockout Validated

FAK is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in FAK knockout HEK293T cell line.

Simple Western

20 µg/mL
Sample: MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line,

Western Blot

2 µg/mL
Sample: MCF-7 human breast cancer cell line, A549 human lung carcinoma cell line, Huh-7 human hepatoma cell line, and HUVEC human umbilical vein endothelial cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using MAB4467 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAK

Focal adhesion kinase 1 (FAK), also known as FAK1 and PTK2, is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in focal adhesions. This cellular localization is directed by a C-terminal 125 amino acid "Focal Adhesion Targeting" (FAT) sequence. FAK plays an important role in migration, cell spreading, differentiation and apoptosis. It associates with several different signaling proteins, such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and Paxillin. These associations enable FAK to function within a network of integrin-stimulated signaling pathways, leading to the activation of targets such as the ERK and JNK mitogen-activated protein kinase pathways. Increased expression and/or activity of FAK in various cancers has been correlated with enhanced proliferation, migration and invasiveness of human tumor cells.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Entrez Gene IDs

5747 (Human); 14083 (Mouse); 25614 (Rat)

Gene Symbol

PTK2

UniProt

Product Documents for Human FAK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FAK Antibody

For research use only

Citations for Human FAK Antibody (1)

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Reconstitution Calculator

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