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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Knockout Validated, Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 495919

Product Specifications

Immunogen

E. coli-derived recombinant human FAK
Asp213-Thr412
Accession # Q05397

Specificity

Detects human FAK in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human FAK Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FAK

Focal adhesion kinase 1 (FAK), also known as FAK1 and PTK2, is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in focal adhesions. This cellular localization is directed by a C-terminal 125 amino acid "Focal Adhesion Targeting" (FAT) sequence. FAK plays an important role in migration, cell spreading, differentiation and apoptosis. It associates with several different signaling proteins, such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and Paxillin. These associations enable FAK to function within a network of integrin-stimulated signaling pathways, leading to the activation of targets such as the ERK and JNK mitogen-activated protein kinase pathways. Increased expression and/or activity of FAK in various cancers has been correlated with enhanced proliferation, migration and invasiveness of human tumor cells.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Entrez Gene IDs

5747 (Human); 14083 (Mouse); 25614 (Rat)

Gene Symbol

PTK2

UniProt

Additional FAK Products

Product Documents for Human FAK Alexa Fluor® 405-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FAK Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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