Human CD45 Antibody

Name: Human CD45 Antibody
Brand: R&D Systems
SKU: MAB1430
Price: 152 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1430

Citations (11)

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Species Reactivity:	Human
Immunogen:	Human peripheral blood mononuclear cells
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Knockout Validated
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 2D1
Specificity:	Detects human CD45. This antibody recognizes all isoforms of human CD45.

View Full Technical Details for Human CD45 Antibody

Applications for Human CD45 Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD45 Antibody

	Flow Cytometry Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
	Immunocytochemistry CD45 in Human PBMCs. CD45 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA blast using Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Flow Cytometry Detection of CD45 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (MAB002), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.
	Knockout Validated CD45 Specificity is Shown by Flow Cytometry in Knockout Cell Line. CD45 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (MAB002) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (F0102B). No staining in the CD45 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

Item	Value
References	Anderson, J.N. et al. (2004) FASEB J. 18:8. Streuli, M. et al. (1987) J. Exp. Med. 166:1548. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608. Ralph, S.J. et al. (1987) EMBO J. 6:1251. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146. McNiell, L. et al. (2007) Immunity 27:425. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911. Zhu, J.W. et al. (2008) Immunity 28:183. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name	Cluster of Differentiation 45
Entrez Gene IDs	5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names	B220, CD45, CD45 antigen, CD45R, CD_antigen: CD45, EC 3.1.3.48, EC:3.1.3.48, GP180, L-CA, LCA, LY5, Leukocyte common antigen, PTPRC, T200, T200 Glycoprotein, T200 leukocyte common antigen, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C

Citations for Human CD45 Antibody (11)

Showing 1 - 10 of 11 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CD45 Antibody include:

Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry

SM Koura et al. (2019), Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration Mol Med Rep, 0(0).
PMID: 30720108
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MJ Costa et al. (2019), Optimal design, anti-tumour efficacy and tolerability of anti-CXCR4 antibody drug conjugates Sci Rep, 9(1):2443.
PMID: 30792442
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

J Yan et al. (2019), FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation Nat Commun, 10(1):448.
PMID: 30683885
Species: Human
Sample Types: Whole Tissue
Applications: IHC

JR Fan et al. (2018), Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model Cell Death Dis, 9(5):502.
PMID: 29717132
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

S Du et al. (2018), Blockade of Tumor-Expressed PD-1 promotes lung cancer growth Oncoimmunology, 7(4):e1408747.
PMID: 29632720
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

ALT Loi et al. (2017), Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients Respir. Res., 18(1):100.
PMID: 28532454
Species: Human
Sample Types: Whole Tissue
Applications: IHC

NS Harasymowi et al. (2017), Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and III Obesity in Patients with OA Arthritis rheumatology (Hoboken, N.J.), 2017;0(0).
PMID: 28320058
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

CL Roberts et al. (2017), Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors Stem Cells Int, 2017(0):1513281.
PMID: 28250775
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016), Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke Stem Cells Int, 2016(0):6104780.
PMID: 27840648
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Keats E et al. (2014), Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells, 32(6):1649-60.
PMID: 24496952
Species: Human
Sample Types: Whole Cells
Applications: ICC

Harvanova D et al. (2011), Isolation and characterization of synovial mesenchymal stem cells. Folia Biol. (Praha), 57(3):119-24.
PMID: 21888835

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Showing 1 - 10 of 11 citations Showing All

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Human CD45 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human CD45 Antibody

Scientific Data Examples for Human CD45 Antibody

Flow Cytometry

Immunocytochemistry

Flow Cytometry

Knockout Validated

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD45

Citations for Human CD45 Antibody (11)

Product Documents for Human CD45 Antibody

Product Datasheets

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