Human CD45 Biotinylated Antibody

Name: Human CD45 Biotinylated Antibody
Brand: R&D Systems
SKU: BAM1430
Price: 411 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAM1430

Citations (7)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Human peripheral blood mononuclear cells
Applications:	Flow Cytometry, Immunocytochemistry/Immunofluorescence
Label:	Biotin
Source:	Monoclonal Mouse IgG₁ Clone # 2D1
Specificity:	Detects human CD45. This antibody recognizes all isoforms of human CD45.

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Applications for Human CD45 Biotinylated Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Human peripheral blood mononuclear cells
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD45 Biotinylated Antibody

	Immunocytochemistry CD45 in human PBMCs. CD45 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD45 Biotinylated Monoclonal Antibody (Catalog # BAM1430) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily
O-glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

Item	Value
References	Anderson, J.N. et al. (2004) FASEB J. 18:8. Streuli, M. et al. (1987) J. Exp. Med. 166:1548. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608. Ralph, S.J. et al. (1987) EMBO J. 6:1251. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146. McNiell, L. et al. (2007) Immunity 27:425. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911. Zhu, J.W. et al. (2008) Immunity 28:183. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name	Cluster of Differentiation 45
Entrez Gene IDs	5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names	B220, CD45, CD45 antigen, CD45R, CD_antigen: CD45, EC 3.1.3.48, EC:3.1.3.48, GP180, L-CA, LCA, LY5, Leukocyte common antigen, PTPRC, T200, T200 Glycoprotein, T200 leukocyte common antigen, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C

Citations for Human CD45 Biotinylated Antibody (7)

Showing 1 - 7 of 7 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CD45 Biotinylated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

JW Franses et al. (2020), Pancreatic circulating tumor cell profiling identifies LIN28B as a metastasis driver and drug target Nat Commun, 11(1):3303.
PMID: 32620742
Species: Human
Sample Types: Whole Blood
Applications: Neutralization

RY Ebright et al. (2020), HIF1A signaling selectively supports proliferation of breast cancer in the brain Nature Communications, 11(1):6311.
PMID: 33298946
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection

X Hong et al. (2018), Molecular signatures of circulating melanoma cells for monitoring early response to immune checkpoint therapy Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 29453278
Species: Human
Sample Types: Whole Blood
Applications: Bioassay

Y Zheng et al. (2017), Expression of ?-globin by cancer cells promotes cell survival during blood-borne dissemination Nat Commun, 8(0):14344.
PMID: 28181495
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016), HER2 expression identifies dynamic functional states within circulating breast cancer cells Nature, 0(0).
PMID: 27556950
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Tseng C et al. (2015), Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin and Staphylococcus aureus Skin Infection. PLoS Pathog, 11(11):e1005292.
PMID: 26618545
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection

Brownell J et al. (0), Independent, parallel pathways to CXCL10 induction in HCV-infected hepatocytes. J Hepato59(4):701-8.
PMID: 23770038

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Showing 1 - 7 of 7 citations Showing All

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Human CD45 Biotinylated Antibody

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Applications for Human CD45 Biotinylated Antibody

Scientific Data Examples for Human CD45 Biotinylated Antibody

Immunocytochemistry

Formulation, Preparation and Storage

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Background: CD45

Citations for Human CD45 Biotinylated Antibody (7)

Product Documents for Human CD45 Biotinylated Antibody

Product Datasheets

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