Human CD45 Phycoerythrin MAb (Clone 2D1)

Name: Human CD45 Phycoerythrin MAb (Clone 2D1)
Brand: R&D Systems
SKU: FAB1430P
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB1430P

Citations (11)

(1)

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Species Reactivity:	Human
Immunogen:	Human peripheral blood mononuclear cells
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 2D1
Specificity:	Detects human CD45. This antibody recognizes all isoforms of human CD45.

View Full Technical Details for Human CD45 Phycoerythrin MAb (Clone 2D1)

Applications for Human CD45 Phycoerythrin MAb (Clone 2D1)

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD45 Phycoerythrin MAb (Clone 2D1)

	Flow Cytometry Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 PE-conjugated Monoclonal Antibody (Catalog # FAB1430P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: CD45

CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the class 1 (Receptor‑like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the Immunological Synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

Item	Value
References	Anderson, J.N. et al. (2004) FASEB J. 18:8. Streuli, M. et al. (1987) J. Exp. Med. 166:1548. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608. Ralph, S.J. et al. (1987) EMBO J. 6:1251. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146. McNiell, L. et al. (2007) Immunity 27:425. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911. Zhu, J.W. et al. (2008) Immunity 28:183. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name	Cluster of Differentiation 45
Entrez Gene IDs	5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names	B220, CD45, CD45 antigen, CD45R, CD_antigen: CD45, EC 3.1.3.48, EC:3.1.3.48, GP180, L-CA, LCA, LY5, Leukocyte common antigen, PTPRC, T200, T200 Glycoprotein, T200 leukocyte common antigen, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C

Citations for Human CD45 Phycoerythrin MAb (Clone 2D1) (11)

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Citations are publications that use Bio-Techne products. Selected citations for Human CD45 Phycoerythrin MAb (Clone 2D1) include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MAA Hussein et al. (2022), Human Wharton's Jelly Mesenchymal Stem Cells Secretome Inhibits Human SARS-CoV-2 and Avian Infectious Bronchitis Coronaviruses Cells, 11(9).
PMID: 35563714
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Liu et al. (2021), Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation BMC Oral Health, 21(1):470.
PMID: 34560867
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

G Belthier et al. (2021), CD44v6 Defines a New Population of Circulating Tumor Cells Not Expressing EpCAM Cancers, 13(19).
PMID: 34638450
Species: Human
Sample Types: Whole Tissue
Applications: IHC

L Jerby-Arno et al. (2021), Opposing immune and genetic mechanisms shape oncogenic programs in synovial sarcoma Nature Medicine, 0(0).
PMID: 33495604
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

BD Jaimes-Par et al. (2017), Membranes derived from human umbilical cord Wharton's jelly stem cells as novel bioengineered tissue-like constructs Histol. Histopathol., 0(0):11897.
PMID: 28444679
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016), Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction PLoS ONE, 11(8):e0160153.
PMID: 27560136
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Monteiro Carvalho Mori da M et al. (2015), Amniotic Fluid Derived Stem Cells with a Renal Progenitor Phenotype Inhibit Interstitial Fibrosis in Renal Ischemia and Reperfusion Injury in Rats. PLoS ONE, 10(8):e0136145.
PMID: 26295710
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Brady K et al. (2014), Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis. Stem Cells Dev, 23(5):541-54.
PMID: 24172175
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kelly MA et al. (2009), Signaling hierarchy regulating human endothelial cell development. Arterioscler. Thromb. Vasc. Biol., 29(5):718-24.
PMID: 19213939
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Baatar D et al. (2007), CCR4-expressing T cell tumors can be specifically controlled via delivery of toxins to chemokine receptors. J. Immunol., 179(3):1996-2004.
PMID: 17641067
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Garzon I et al. (0), Wharton's jelly stem cells: a novel cell source for oral mucosa and skin epithelia regeneration. Stem Cells Transl Me2(8):625-32.
PMID: 23817131

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Customer Reviews for Human CD45 Phycoerythrin MAb (Clone 2D1) (1)

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Human CD45 PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: Human embryonic stem cells (H9 from Wicell)

Species: Human

Verified Customer | Posted 08/16/2016

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Product Documents for Human CD45 Phycoerythrin MAb (Clone 2D1)

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Human CD45 Phycoerythrin MAb (Clone 2D1)

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human CD45 Phycoerythrin MAb (Clone 2D1)

Scientific Data Examples for Human CD45 Phycoerythrin MAb (Clone 2D1)

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: CD45

Citations for Human CD45 Phycoerythrin MAb (Clone 2D1) (11)

Customer Reviews for Human CD45 Phycoerythrin MAb (Clone 2D1) (1)

Human CD45 PE-conjugated Antibody

Product Documents for Human CD45 Phycoerythrin MAb (Clone 2D1)

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