Human BLAME/SLAMF8 Antibody

Name: Human BLAME/SLAMF8 Antibody
Brand: R&D Systems
SKU: MAB19072

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB19072

Citations (3)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8 Ala23-Asp233 Accession # Q9P0V8
Applications:	CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 250014
Specificity:	Detects human BLAME/SLAMF8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SLAMF7 or rhSLAMF6 is observed.

View Full Technical Details for Human BLAME/SLAMF8 Antibody

Applications for Human BLAME/SLAMF8 Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human BLAME/SLAMF8 Antibody

	Flow Cytometry Detection of BLAME/SLAMF8 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were treated for with Recombinant Human IFN-gamma (Catalog # 285-IF) then stained with Mouse Anti-Human BLAME/SLAMF8 Monoclonal Antibody (Catalog # MAB19072, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: BLAME/SLAMF8

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19%‑26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).

Item	Value
References	McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91. Veillette, A. (2006) Nat. Rev. Immunol. 6:56. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.
Long Name	SLAM Family Member 8
Entrez Gene IDs	56833 (Human); 74748 (Mouse); 289237 (Rat); 102122568 (Cynomolgus Monkey)
Alternate Names	B Lymphocyte Activator Macrophage Expressed, B-Lymphocyte Activator Macrophage Expressed, BCM-Like Membrane Protein, BLAME, CD353, CD353 Antigen, SBBI42, SLAM Family Member 8, SLAMF8

Citations for Human BLAME/SLAMF8 Antibody (3)

Showing 1 - 3 of 3 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human BLAME/SLAMF8 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016), A combination of an anti-SLAMF6 antibody and ibrutinib efficiently abrogates expansion of chronic lymphocytic leukemia cells Oncotarget, 7(18):26346-60.
PMID: 27029059
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Matesanz-Isabel J et al. (2011), New B-cell CD molecules. Immunol. Lett., 134(2):104-12.
PMID: 20933010
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Cabezon R et al. (2011), Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 134(2):167-73.
PMID: 20933012

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Showing 1 - 3 of 3 citations Showing All

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Human BLAME/SLAMF8 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human BLAME/SLAMF8 Antibody

Scientific Data Examples for Human BLAME/SLAMF8 Antibody

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: BLAME/SLAMF8

Citations for Human BLAME/SLAMF8 Antibody (3)

Product Documents for Human BLAME/SLAMF8 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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