Western blot shows lysates of A431 human epithelial carcinoma cell line and human cerebeullum tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Adenosine A2b R Monoclonal Antibody (Catalog # MAB10444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Adenosine A2b R at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Adenosine A2b R Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: A431 human epithelial carcinoma cell line and Human cerebeullum tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2b R

ADORA2B (Adenosine B2a Receptor, Adora-B2) is a widely expressed seven transmembrane G protein-coupled receptor (GPCR) activated by adenosine. Studies show that adenosine is crucial for ischemic preconditioning (IP) via its receptors ADORA1, ADORA2a and ADORA2b. All of these receptors are expressed in the myocardium and have been implicated in the role of cardioprotection during ischemia. Human and mouse Adora2B sequences are 88% identical over the full protein, but only 77% identical on the extra-cellular domains, including N-term tail and three loops.

Long Name

Adenosine A2b Receptor

Alternate Names

Adenosine A2bR, ADORA2B

Entrez Gene IDs

136 (Human); 11541 (Mouse); 29316 (Rat)

Gene Symbol

ADORA2B

Additional Adenosine A2b R Products

Product Documents for Human Adenosine A2b R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Adenosine A2b R Antibody

For research use only

Citations
Reviews

Human Adenosine A2b R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10444

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Adenosine A2b R Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Adenosine A2b R Antibody

Detection of Human Adenosine A2b R by Western Blot.