Detects human 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB, recombinant human (rh) CD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLTR beta, rmLTR beta, rhNGFR, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human 4-1BB/TNFRSF9/CD137 Antibody

Detection of 4‑1BB/TNFRSF9/ CD137 in Human CD3⁺PBMCs by Flow Cytometry.

Human CD3⁺peripheral blood mononuclear cells were treated for 48 hours with 5 µg/mL PHA then stained with Mouse Anti-Human 4-1BB/TNFRSF9/CD137 Mono-clonal Antibody (Catalog # MAB838, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Applications for Human 4-1BB/TNFRSF9/CD137 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human CD3⁺ peripheral blood mononuclear cells treated with PHA

Western Blot

1 µg/mL
Sample: Recombinant Human 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 838-4B)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB/TNFRSF9/CD137

4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 KD glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.

References

Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.

Alternate Names

41BB, CD137, ILA, TNFRSF9

Entrez Gene IDs

3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)

Gene Symbol

TNFRSF9

UniProt

Q07011

Additional 4-1BB/TNFRSF9/CD137 Products

Product Documents for Human 4-1BB/TNFRSF9/CD137 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human 4-1BB/TNFRSF9/CD137 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human 4-1BB/TNFRSF9/CD137 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB838

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all 4-1BB/TNFRSF9/CD137 Antibodies »

Product Specifications

Immunogen

Specificity