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Simple Western Sized-Based Assay Data

Simple Western protein gel

What Does Size-Based Simple Western Assay Data Look Like?

Simple Western size-based assay data is processed automatically in Compass for SW for you. Sample data is displayed by lane in a virtual blot-like image similar to traditional results with one big exception—not only do you get more information, but you also get it as soon as the assay is complete. Quantitative results such as molecular weight, signal intensity (area), % area, and signal-to-noise for each immunodetected protein are presented in the results table automatically.

If you're more familiar with capillary electrophoresis, you can see your results in a more traditional electropherogram view too.

Simple Western size-based electropherogram
Simple Western protein panels
Panel of proteins run using both a 12–230 kDa (A) and a 66–440 kDa (B) pre-filled plate.

Detectable Molecular Weight Range

With kits offering sizing ranges from 2 kDa to 440kDa, Simple Western assays support the detection of varying protein sizes.

Separation module molecular weight ranges:

  • 2-40 kDa
  • 12 – 230 kDa
  • 66 – 440 kDa

Reproducible, Quantitative Size-Based Data

Our Simple Western systems give you the detection and quantitation you need, whether you’re looking for absolute or relative quantitation or wanting to simultaneously quantitate high and low protein concentrations.

Simple Western assays are fully automated by JessAbbyWesPeggy Sue, or Sally Sue and easily standardized, making your results much more reproducible. And there’s no blotting step, so inconsistencies caused by protein transfer are eliminated, which really improves your quantitation too!

 

Figure (right): Phosphoinositide 3-kinase (PI3K) expression in an MCF-7 lysate. Ten replicates (n=10) of MCF7 lysate at 1 µg/µL were analyzed using a PI3K-specific primary antibody. The predicted molecular weight of PI3K is 110 kDa. Simple Western assay data reported an average molecular weight of 107 kDa with a 0.5% CV for sizing and an 8.7% CV for signal intensity (area).

Phosphoinositide 3-kinase (PI3K) expression in an MCF-7 lysate blot
Yotis Senis Thumbnail

From Your Peers

“[Wes] Provides quantitative data, saves time and uses far less sample.” - Yotis Senis, Ph.D., Professor and Chair, University of Birmingham, UK

Assay Dynamic Range

Simple Western assays have a linear dynamic range of approximately 3 orders of magnitude, letting you detect proteins over a wider concentration range, not to mention with more accurate quantitation

Dynamic range of detection for GSK-3 alpha graph