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Are You Looking for Reliable Quantitative Results?

  • Are you looking for reliable absolute or relative quantitation of your target?
  • Do you want to simultaneously detect high and low protein concentrations?

Quantitative Results with Simple Western

Our Simple Western™ systems give you the detection and quantitation you need for reliable results. Reliably detect signal saturation and work within the linear dynamic range of your assay. With HDR detection profile and analysis Simple Western gets you up to 6 logs of dynamic range. Get quantitative, reproducible results with Simple Western.

Generating quantitative dose-response curves with Simple Western instruments Jess and Peggy Sue

Strengthen Dose Response Curves

Simple Western is an ideal platform for dose-response measurements in drug development studies. Reveal changes in target abundance, target degradation, or post-translational modifications with:

  • High throughput
  • Speed
  • Sensitivity
  • Accuracy
  • Minimal matrix effects

Simple Western Charge and Size assays deliver accurate dose response measurements in a variety of applications including pharmacokinetic assays and targeted protein degradation.

Protac dose response curve with Jess

 

From Your Peers in Drug Discovery

"I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently."

- Rachel Doidge Ph.D, Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK

Rachel Doidge thumbnail

High Sensitivity Multiplex

Quantify several targets at once with chemiluminescence and industry-leading NIR/IR fluorescence detection sensitivity. Stellar NIR/IR Modules™ for Jess can accurately quantify low-abundance targets by leveraging multi-color fluorescence and chemiluminescence multiplex.

RePlex™ Modules for Jess and Abby perform sequential immunoassay or total protein detectionLower the cost per run and further increase the number of quantitative results from your sample.

Industry-leading NIR/IR fluorescence detection sensitivity with Stellar

Automated Protein Normalization

Housekeeping controls are unreliable controls for protein normalization. Normalizing target protein expression data by total protein is the only reliable and accurate method to understand how protein expression changes as cells respond to their environment, and scientific journals are increasingly demanding it. Simple Western has several different methods for automated total protein normalization for publication-ready results. Jess has a dedicated fluorescence channel for total protein normalization. Just add the Protein Normalization Reagent and Jess will normalize your protein expression data of targets detected in chemiluminescence and NIR/IR fluorescence channels.

Jess Superplex Chemiluminescence Fluorescence Graph

Quantitative Analysis with Compass

Raw and normalized results automatically appear in Simple Western instrument software Compass for Simple Western.

Jess Superplex Compass Table