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ExCellerate™ Serum-Free Cell Expansion Media
Take an important step towards defined cell culture and improve the consistency of your cell therapy process. ExCellerate media provide an optimized serum-free culture environment for the generation and expansion of individual immune cell and stem cell types.
- Reduce variability in your media composition
- Streamline comparability testing if raw material changes are required
- Facilitate regulatory compliance during clinical transition
- Reduce time-to-market for your cell therapy
How Do We Define Xeno-Free and Animal Component-Free Culture Media?
- Xeno-free media do not contain any non-human animal-derived components. Xeno-free media may contain human-derived components such as serum, holo-transferrin, or insulin.
- Animal component-free media do not contain any animal- or human-derived components. Elimination of these components further improves culture consistency compared to xeno-free media.
Eliminate variability from trace animal components in your culture media and reduce the risk of inconsistent results. Cultures expanded in these media are unaffected by the presence of unknown levels of vitamins, hormones, and growth factors in serum.
- Optimized for use with R&D Systems™ cytokines and growth factors
- Compatible with a variety of culture and activation methods
We thoroughly test each production lot of ExCellerate media for the presence of animal components, and this documentation is available on request.
ExCellerate Human T Cell Expansion Media
- Robust T cell expansion from PBMCs or purified CD3+ T cells
- Optimized for use with Cloudz™ T Cell Activation Kits
ExCellerate Human NK Cell Expansion Media
- Robust feeder-free expansion from PBMCs or isolated NK cells
- Compatible with a variety of NK cell activation methods
ExCellerate B Cell Media
- Robust B cell expansion of B cells from peripheral blood in multiple species
- Supports antigen-specific B cell clonogenicity and hybridoma culture
ExCellerate iPSC Expansion Media
- Robust feeder-free expansion of undifferentiated cells
- Complete formulation; no growth factor supplementation required
- Maintains genetic stability and differentiation potential over long-term culture
- Compatible with several matrices and cell lines
Transitioning Research to GMP
As you advance from discovery into process development and clinical manufacturing, we make the transition as efficient and seamless as possible. Our GMP materials are based on our research-grade products wherever possible. If GMP-grade materials are not available, our Custom Services team will work with you to convert RUO materials into products appropriate for manufacturing use. We have recently developed an intermediate tier of small molecules to meet the rapidly increasing needs of the cell therapy industry.
Our Ancillary Materials Grade Small Molecules are certified animal-free and supported by enhanced QC testing, a more detailed QA review, and accompanying documentation. Incorporation of GMP or AM-grade raw materials early in your program will simplify your clinical transition. Our diligence with quality control ensures that you can move seamlessly into regulatory agency submissions without disrupting your process.
Cloudz™ Cell Activation Kits
Cloudz™ Cell Activation Kits
Cloudz reagents are microspheres composed of an alginate-based hydrogel. The microspheres can be functionalized with a variety of ligands including antibodies, proteins, and small molecules to activate desired cells and enable cell expansion.
Exposure of immune cells to functionalized Cloudz microspheres triggers intracellular signaling to promote cell expansion and robust cell culture.
The alginate hydrogel microspheres quickly dissolve when exposed to our proprietary Release Buffer supplied in the kits, allowing for gentle removal of the microspheres. This dissolving property also allows you to precisely and rapidly remove residual Cloudz and their corresponding ligands from the expanded cells.
ScaleReady™
ScaleReady™
Bio-Techne believes in the power of partnerships. We have joined with Fresenius Kabi and Wilson Wolf to bring you ScaleReady for immune cell therapy manufacturing.
ScaleReady brings cell and gene therapies to life by streamlining manufacturing processes across cell culture, cell activation and expansion, gene editing, and cell processing.
ExCellerate T cell and NK cell expansion media for immune cell therapy applications are available through ScaleReady. For all other applications, ExCellerate media are available through Bio-Techne.
Website Resources
- Immune Cell Culture
- Stem Cells
- Organoids and 3D Culture
- Gene Engineering Services
- Transitioning to Animal-Free and GMP
- GMP Capabilities
- Cell and Gene Therapy
- Custom Cell and Gene Therapy Services
- Cell Culture Products
- Fetal Bovine Serum
- Cultrex™ Basement Membrane Extracts and Extracellular Matrix Proteins
- Fluorokines™ Fluorescent-Labeled Recombinant Proteins at R&D Systems
- Serum-Free and Animal-Free Cell Culture
- Cloudz™ Cell Activation Kits
Literature
- T Cell-Based Therapies eBook
- Immune Cell Protocols at R&D Systems
- Immune Cell Isolation & Culture Brochure at R&D Systems
- Stem Cell Protocols at R&D Systems
- The Organoid Culture Handbook at R&D Systems
- Evolution of Cell Culture Model Systems eBook at R&D Systems
- The Importance of Serum- and Animal-Free Media for Stem Cell-Based Therapeutics Article at RegMedNet
ExCellerate media are formulated and optimized for the ex vivo culture of immune cells and stem cells. Unlike traditional serum-containing culture media, these media provide a stable culture and optimized environment, devoid of non-human animal-derived products. ExCellerate iPSC medium does not contain any animal-derived or human-derived products. ExCellerate media support the expansion of specific cell types (T cells, NK cells, B cells, or pluripotent stem cells)
