Pyridone 6
Tocris Bioscience, part of Bio-Techne | Catalog # 6577
Potent pan-JAK inhibitor; induces intermediate mesoderm; cell-permeable
Key Product Details
Description
Potent pan-JAK inhibitor; induces intermediate mesoderm; cell-permeable
Alternative Names
JAK inhibitor I
Product Description
Pyridone 6 is a potent pan-JAK inhibitor; ATP-competitive inhibitor of JAK 1/2/3 and Tyk2 (IC50 values are 1, 5, 15 and 1 nM, respectively). Inhibits other kinases tested at 130 nM to >10 μM. Inhibits IL-2- and IL-4-dependent proliferation of CTLL cells and blocks STAT5 phosphorylation. Also inhibits Th1 and Th2 development, and promotes Th17 differentiation from naive T cells. When used in combination with Retinoic acid, LY 294002 and CCG 1423, it induces intermediate mesoderm differentiation from ESCs. Inhibits growth of primary myeloma cells grown in the presence of bone marrow stromal cells. Cell-permeable.Product Specifications
Molecular Weight
309.34
Formula
C18H16FN3O
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
2-(1,1-Dimethylethyl)-9-fluoro-1,6-dihydro-7H-benz[h]imidazo[4,5-f]isoquinolin-7-one
CAS Number
457081-03-7
PubChem ID
5494425
InChI Key
VNDWQCSOSCCWIP-UHFFFAOYSA-N
SMILES
CC(C)(C1=NC2=C3C=CNC(C3=C4C=C(C=CC4=C2N1)F)=O)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.93 | 100 | |
| Ethanol | 15.47 | 50 |
Preparing Stock Solutions
for Pyridone 6
The following data is based on the product molecular weight 309.34
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.23 mL | 16.16 mL | 32.33 mL |
| 5 mM | 0.65 mL | 3.23 mL | 6.47 mL |
| 10 mM | 0.32 mL | 1.62 mL | 3.23 mL |
| 50 mM | 0.06 mL | 0.32 mL | 0.65 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Thompson Photochemical preparation of a pyridone containing tetracycle: a Jak protein kinase inhibitor. Bioorg.Med.Chem.Lett. 2002 PMID: 11934592
- Mae Combination of small molecules enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells. Biochem.Biophys.Res.Commun. 2010 PMID: 20171952
Product Specific Notices for Pyridone 6
For research use only
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