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Recombinant SARS-CoV-2 P.1 S1 NTD His-tag Protein, CF

Catalog # 11111-CV | R&D Systems, Inc. a Bio-Techne Brand
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11111-CV-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike S1 Subunit protein
Val16-Leu303 (Leu18Phe, Thr20Asn, Pro26Ser, Asp138Tyr, Arg190Ser), with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val16

Predicted Molecular Mass

34 kDa

SDS-PAGE

57-63 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant SARS-CoV-2 P.1 S1 NTD His-tag Protein, CF

Recombinant SARS-CoV-2 P.1 Spike S1 NTD His-tag Protein SDS-PAGE

2 μg/lane of Recombinant SARS-CoV-2 P.1 Spike S1 NTD His-tag Protein (Catalog # 11111-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-63 kDa.

Formulation, Preparation and Storage

11111-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike S1 Subunit

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS‑CoV and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into S1 and S2 subunits is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor, while the S2 subunit is involved with cell fusion (3-5). The S1 subunit can be further divided into an N-terminal domain (NTD) and a receptor binding domain (RBD). The SARS-CoV-2 NTD shares 50% and 20% amino acid (aa) sequence identity with the NTD of SARS-CoV-1 and MERS-CoV, respectively. The NTD is reported to bind L-SIGN and DC-SIGN in cells that don't express the ACE-2 receptor (6). Despite being heavily glycosylated, the NTD is capable of eliciting an immune response to produce potent neutralization antibodies, although at a reduced level than the ones targeting the RBD. Three immunogenic regions have been identified in the NTD: aa 14-20, aa 140-158, and aa 245-264 (7). Antibody cocktails targeting both NTD and RBD could provide better protection against SARS-CoV-2 infection. There are five mutations in the NTD of P.1 variant.

References

  1. Wu, F. et al. (2020) Nature 579:265.
  2. Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93.
  3. Bosch, B.J. et al. (2003) J. Virol. 77:8801.
  4. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
  5. Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.
  6. Soh, W.T. et al. (2020) bioRxiv doi: https://doi.org/10.1101/2020.11.05.369264.
  7. Matthew, M. et al. (2021) Cell 184:2332.

Long Name

Spike Protein, S1 Subunit

UniProt

Additional Spike S1 Subunit Products

Product Documents for Recombinant SARS-CoV-2 P.1 S1 NTD His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant SARS-CoV-2 P.1 S1 NTD His-tag Protein, CF

For research use only

Reconstitution Calculator

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