Recombinant SARS-CoV-2 E484K N501Y Spike RBD His Protein, CF
Catalog # 10788-CV | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Arg319-Phe541 (Glu484Lys, Asn501Tyr), with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Scientific Data Images for Recombinant SARS-CoV-2 E484K N501Y Spike RBD His Protein, CF
Recombinant SARS-CoV-2 E484K N501Y Spike RBD His-tag Protein Binding Activity.
Recombinant SARS-CoV-2 E484K N501Y Spike RBD His-tag (Catalog # 10788-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA.Recombinant SARS-CoV-2 E484K N501Y Spike RBD His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant SARS-CoV-2 E484L N501Y Spike RBD His-tag (Catalog # 10788-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-40 kDa.Formulation, Preparation and Storage
10788-CV
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Spike RBD
References
- Wu, F. et al. (2020) Nature 579:265.
- Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93.
- Bosch, B.J. et al. (2003). J. Virol. 77:8801.
- Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
- Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.
- Li, W. et al. (2003) Nature 426:450.
- Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
- Kozlov, Max (2020) TheScientist https://www.the-scientist.com/news-opinion/new-sars-cov-2-variant-spreading-rapidly-in-uk-68292.
- Wise, J. (2020) B.M.J 371:m4857.
- Tang, J.W. et al. (2020) J. Infect. doi: 10.1016/j.jinf.2020.12.024.
- Davies, N.G. (2020) medRxiv doi:10.1101/2020.12.24.20248822.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant SARS-CoV-2 E484K N501Y Spike RBD His Protein, CF
Product Specific Notices for Recombinant SARS-CoV-2 E484K N501Y Spike RBD His Protein, CF
For research use only
Reconstitution Calculator
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