Recombinant SARS-CoV-2 BA.4/BA.5 S1 NTD His-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 11183-CV
Omnicron Variant
Key Product Details
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike S1 Subunit protein
Val16-Leu303 (Thr19Ile, Leu24del, Pro25del, Pro26del, Ala27Ser, His69del, Val70del, Gly142Asp, Val213Gly), with a C-terminal 6-His tag
Val16-Leu303 (Thr19Ile, Leu24del, Pro25del, Pro26del, Ala27Ser, His69del, Val70del, Gly142Asp, Val213Gly), with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val16
Predicted Molecular Mass
33 kDa
SDS-PAGE
53-59 kDa, under reducing conditions.
Activity
Bioassay data are not available.
Scientific Data Images for Recombinant SARS-CoV-2 BA.4/BA.5 S1 NTD His-tag Protein, CF
Recombinant SARS-CoV-2 BA.4/BA.5 Spike S1 Subunit NTD His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant SARS-CoV-2 BA.4/BA.5 Spike S1 Subunit NTD His-tag Protein (Catalog # 11183-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-59 kDa.Formulation, Preparation and Storage
11183-CV
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Spike S1 Subunit
References
- Wu, F. et al. (2020) Nature 579:265.
- Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93.
- Bosch, B.J. et al. (2003) J. Virol. 77:8801.
- Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
- Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.
- Soh, W.T. et al. (2020) bioRxiv doi: https://doi.org/10.1101/2020.11.05.369264.
- McCallum, M. et al. (2021) Cell 184:2332.
Long Name
Spike Protein, S1 Subunit
Alternate Names
SARS-CoV-2
UniProt
Additional Spike S1 Subunit Products
Product Documents for Recombinant SARS-CoV-2 BA.4/BA.5 S1 NTD His-tag Protein, CF
Product Specific Notices for Recombinant SARS-CoV-2 BA.4/BA.5 S1 NTD His-tag Protein, CF
For research use only
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