Recombinant Rat TIM-3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10489-TM

R&D Systems, part of Bio-Techne
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10489-TM-050
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Key Product Details

Source

NS0

Accession #

P0C0K5.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TIM-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat TIM-3 protein
Rat TIM-3
(Leu22-Ala194)
Accession # P0C0K5.2		 IEGRMD Mouse IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22

Predicted Molecular Mass

46 kDa

SDS-PAGE

60-75 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Galectin-9 Protein  (Catalog # 2045-GA) is immobilized at 1.0 µg/mL (100 µL/ well), Recombinant Rat TIM‑3 Fc Chimera (Catalog # 10489-TM) that produces a 50% optimal binding response is found to be 20-120 ng/mL

Scientific Data Images for Recombinant Rat TIM-3 Fc Chimera Protein, CF

Recombinant Rat TIM-3 Fc Chimera Protein Bioactivity

Recombinant Rat TIM-3 Fc Chimera Protein Bioactivity

When Recombinant Human Galectin-9 Protein (2045-GA) is immobilized 1.0 μg/mL (100 μL/well), Recombinant Rat TIM-3 Fc Chimera (10489-TM) that produces a 50% optimal binding response is found to be 20-120 ng/mL.
Recombinant Rat TIM-3 Fc Chimera Protein SDS-PAGE

Recombinant Rat TIM-3 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Rat TIM-3 Fc Chimera Protein (10489-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa and 120-150 kDa, respectively.

Formulation, Preparation and Storage

10489-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-3

T cell immunoglobulin and mucin domain-3 (TIM-3) also known as HAVCR2, is a member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain, a Ser/Thr-rich mucin stalk region (1, 2). While lacking a specific immunoreceptor tyrosine-based inhibition motif (ITIM), the cytoplasmic domain of TIM-3 contains a conserved region of five tyrosine residues important for downstream signaling (3). A soluble form of TIM-3 lacking the mucin stalk and transmembrane domains is formed as either a result of alternative splicing or metalloproteinase-dependent cleavage. Within the ECD, mature rat TIM-3 shares 60% and 69% amino acid sequence identity with human and mouse TIM-3, respectively. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (3,11). TIM-3 ligation attenuates CD8+ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

References

  1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
  2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
  3. Wolf, Y. et al. (2020) Nature Reviews Immunology 20:173.
  4. Nakayama, M. et al. (2009) Blood 113:3821.
  5. Anderson, A.C. et al. (2007) Science 318:1141.
  6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
  8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
  10. Gleason, M.K. et al. (2012) Blood 119:3064.
  11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
  13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.

Long Name

T Cell Immunoglobulin Mucin-3

Alternate Names

CD366, HAVcr-2, HAVCR2, KIM-3, SPTCL, TIM3, TIMD3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)

Gene Symbol

HAVCR2

UniProt

P0C0K5.2

Additional TIM-3 Products

Product Documents for Recombinant Rat TIM-3 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Rat TIM-3 Fc Chimera Protein, CF

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