Recombinant Rat CX3CL1/Fractalkine Protein

Name: Recombinant Rat CX3CL1/Fractalkine Protein
Brand: R&D Systems
SKU: 536-FR
Price: 377 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 536-FR

Citations (3)

Product Datasheet / COA / SDS

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	O55145
Applications:	Bioactivity

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Product Specifications for Recombinant Rat CX3CL1/Fractalkine Protein

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat CX3CL1/Fractalkine protein

Rat CX3CL1/Fractalkine (Gln25-Thr334) Accession # O55145	IEGR	GGGSGGGSGGGS	HHHHHHHHHH
N-terminus			C-terminus

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained: Gln25 predicted

Predicted Molecular Mass

36 kDa

SDS-PAGE

100 kDa, reducing conditions

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED₅₀ for this effect is 0.02-0.08 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

536-FR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

536-FR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X₃-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Rat CX3CL1 cDNA encodes a 393 amino acid (aa) residue precursor protein with two alternative (21 aa or 24 aa residue) putative signal peptides, a 74 aa or 76 aa residue globular chemokine domain, a 238 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 36 aa residue cytoplasmic domain. The extracellular domain of CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, rat CX3CL1 shares a high degree of amino acid sequence homology (83% sequence identity) with human and mouse CX3CL1. CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T‑lymphocytes but not monocytes. CX3CR1, previously named V28 or chemokine beta receptor‑like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind CX3CL1 with high-affinity.

Item	Value
References	Kledal, T.N. et al. (1998) FEBS Lett. 441:209. Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799. Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896. Rossi, D.L. et al. (1998) Genomics 47:163.
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names	ABCD-3, C3Xkine, CX3C membrane-anchored chemokine, CX3CL1, CXC3, CXC3C, FKN, Fractalkine, NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, Neurotactin, SCYD1C-X3-C motif chemokine 1, chemokine (C-X3-C motif) ligand 1, neurotactin), small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Recombinant Rat CX3CL1/Fractalkine Protein (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat CX3CL1/Fractalkine Protein include:

Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Kiyomoto M et al. (2013), Fractalkine signaling in microglia contributes to ectopic orofacial pain following trapezius muscle inflammation. J Neurosci, 33(18):7667-80.
PMID: 23637160
Species: N/A
Sample Types: N/A
Applications: Western Blot

Cook A et al. (2010), Interactions between chemokines: regulation of fractalkine/CX3CL1 homeostasis by SDF/CXCL12 in cortical neurons. J. Biol. Chem., 285(14):10563-71.
PMID: 20124406
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Owolabi SA et al. (2006), Fractalkine and minocycline alter neuronal activity in the spinal cord dorsal horn. FEBS Lett., 580(18):4306-10.
PMID: 16842787

Recombinant Rat CX3CL1/Fractalkine Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Rat CX3CL1/Fractalkine Protein

Formulation, Preparation and Storage

Carrier Free

536-FR

Carrier

536-FR/CF

Carrier Free

Reconstitution Calculator

Background: CX3CL1/Fractalkine

Citations for Recombinant Rat CX3CL1/Fractalkine Protein (3)

Product Documents for Recombinant Rat CX3CL1/Fractalkine Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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