Chinese Hamster Ovary cell line, CHO-derived porcine PD-L1/B7-H1 protein Phe19-Thr237, with a C-terminal 6-His tag.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
35-55 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Porcine PD-L1/B7-H1 His-tag (Catalog # 10361-B7) is immobilized at 1 μg/mL (100 µL/well), the concentration of Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 µg/mL.
Recombinant Porcine PD-L1/B7-H1 His-tag Protein, CF Scientific Data Examples
Recombinant Porcine PD-L1/B7-H1 His-tag Protein Binding Activity
When Recombinant Porcine PD-L1/B7-H1 His-tag Protein (Catalog # 10361-B7) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 µg/mL.
Recombinant Porcine PD-L1/B7-H1 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Porcine PD-L1/B7-H1 His-tag (Catalog # 10361-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-55 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Programmed cell death 1 ligand 1 (PD-L1), also known as B7-H1 and CD274, is a transmembrane glycoprotein and a member of the B7 family of immune regulatory molecules within the immunoglobulin (Ig) superfamily (1). Mature porcine PD-L1 consists of an extracellular domain (ECD) with both an Ig-V and Ig-C-like motif, a single transmembrane domain, and a short cytoplasmic tail lacking a canonical signaling motif (1). The mature ECD of porcine PD-L1 shares 74% and 67% amino acid sequence identity with human and mouse B7-H1, respectively. Alternative splicing of PD-L1 generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (2). B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, endothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma (3-9). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7-9). It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells (6, 10). It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development (9). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells (5, 10). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (3-4, 7, 9).
Sun, C. et al. (2018) Immunity. 48:434.
Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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