Recombinant Porcine PD-1 His-tag Protein, CF

Name: Recombinant Porcine PD-1 His-tag Protein, CF
Brand: R&D Systems
SKU: 10432-PD
Price: 504 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10432-PD

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Key Product Details

Source:	CHO
Accession #:	NP_001191308.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Porcine PD-1 His-tag Protein, CF

Product Specifications for Recombinant Porcine PD-1 His-tag Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived porcine PD-1 protein Leu25-Gln169, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu25
Predicted Molecular Mass	17 kDa
SDS-PAGE	36-48 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Porcine B7-H1 Fc Chimera (Catalog # 10397-B7) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Porcine PD‑1 His-tag (Catalog # 10432-PD) that produces 50% of the optimal binding response is approximately 1-6 μg/mL.

Scientific Data Examples for Recombinant Porcine PD-1 His-tag Protein, CF

	Recombinant Porcine PD-1 His-tag Protein Binding Activity When Recombinant Porcine B7-H1 Fc Chimera (Catalog # 10397-B7) is immobilized at 1 µg/mL, 100 µL/well, the concentration of Recombinant Porcine PD-1 His-tag (Catalog # 10432-PD) that produces 50% of the optimal binding response is approximately 1-6 µg/mL.
	Recombinant Porcine PD-1 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Porcine PD-1 His-tag Protein (Catalog # 10432-PD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-48 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10432-PD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. The extracellular domain (ECD) of porcine PD-1 shares 68% and 60% amino acid sequence identity with the human and rat PD-1, respectively. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD-1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and on non-hematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1: PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12-14).

Item	Value
References	Ishida, Y. et al. (1992) EMBO J. 11:3887. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265. Watanabe, N. et al. (2003) Nat. Immunol. 4:670. Zhang, X. et al. (2004) Immunity 20:337. Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483. Nishimura, H et al. (1996) Int. Immunol. 8:773. Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677. Butte, M.J. et al. (2007) Immunity 27:111. Okazaki, T. et al. (2013) Nat. Immunol. 14:1212. Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99:12293. Nogrady, B. (2014) Nature 513:S10. Swaika, A. et al. (2015) Mol. Immunol. 67:4.
Long Name	Programmed Death-1
Entrez Gene IDs	5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names	CD279, CD279 antigen, PD-1, PD1, PD1hPD-l, PDCD1, Protein PD-1, SLEB2, hPD-1, programmed cell death 1, programmed cell death protein 1

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Recombinant Porcine PD-1 His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Porcine PD-1 His-tag Protein, CF

Scientific Data Examples for Recombinant Porcine PD-1 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PD-1

Citations for Recombinant Porcine PD-1 His-tag Protein, CF

Product Documents for Recombinant Porcine PD-1 His-tag Protein, CF

Product Datasheets

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