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Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10397-B7

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10397-B7-100

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived porcine PD-L1/B7-H1 protein
Porcine PD-L1/B7-H1
(Phe19-Thr237)
Accession # NP_001020392.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe19

Predicted Molecular Mass

52 kDa

SDS-PAGE

65-85 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Porcine PD-L1/B7-H1 Fc Chimera (Catalog # 10397-B7) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Porcine PD-1 His-tag that produces 50% of the optimal binding response is found to be approximately 0.8-6 μg/mL.

Scientific Data Images for Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein, CF

Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein Binding Activity

Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein Binding Activity

When Recombinant PD-L1/B7-H1 Fc Chimera (Catalog # 10397-B7) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Porcine PD-1 His-tag that produces 50% of the optimal binding response is found to be approximately 0.8-6 μg/mL.
Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein SDS-PAGE

Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Porcine PD-L1/B7-H1 Fc Chimera (Catalog # 10397-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-85 kDa and 130-170 kDa, respectively.

Formulation, Preparation and Storage

10397-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

Programmed cell death 1 ligand 1 (PD-L1), also known as B7-H1 and CD274, is a transmembrane glycoprotein and a member of the B7 family of immune regulatory molecules within the immunoglobulin (Ig) superfamily (1). Mature porcine PD-L1 consists of an extracellular domain (ECD) with both an Ig-V and Ig-C-like motif, a single transmembrane domain, and a short cytoplasmic tail lacking a canonical signaling motif (1). The mature ECD of porcine PD-L1 shares 74% and 67% amino acid sequence identity with human and mouse B7-H1, respectively. Alternative splicing of PD-L1 generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (2). B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, endothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma (3-9). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7-9). It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells (6, 10). It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development (9). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells (5, 10). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (3-4, 7, 9).

References

  1. Sun, C. et al. (2018) Immunity. 48:434.
  2. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
  3. Kuang, D.M. et al. (2014) J. Clin. Invest. 124:4657.
  4. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  5. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  6. Dong, H. et al. (2002) Nat. Med. 8:793.
  7. Azuma, T. et al. (2008) Blood 111:3635.
  8. Park, J.J. et al. (2010) Blood 116:1291.
  9. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  10. Herold, M. et al. (2015) J. Immunol. 195:3584.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Additional PD-L1/B7-H1 Products

Product Documents for Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein, CF

For research use only

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