Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein, CF

Name: Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10397-B7

Catalog # 10397-B7

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	CHO
Accession #:	NP_001020392
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived porcine PD-L1/B7-H1 protein

Porcine PD-L1/B7-H1 (Phe19-Thr237) Accession # NP_001020392.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe19

Predicted Molecular Mass

52 kDa

SDS-PAGE

65-85 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Porcine PD-L1/B7-H1 Fc Chimera (Catalog # 10397-B7) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Porcine PD-1 His-tag that produces 50% of the optimal binding response is found to be approximately 0.8-6 μg/mL.

Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein Binding Activity When Recombinant PD-L1/B7-H1 Fc Chimera (Catalog # 10397-B7) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Porcine PD-1 His-tag that produces 50% of the optimal binding response is found to be approximately 0.8-6 μg/mL.
	Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Porcine PD-L1/B7-H1 Fc Chimera (Catalog # 10397-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-85 kDa and 130-170 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10397-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PD-L1/B7-H1

Programmed cell death 1 ligand 1 (PD-L1), also known as B7-H1 and CD274, is a transmembrane glycoprotein and a member of the B7 family of immune regulatory molecules within the immunoglobulin (Ig) superfamily (1). Mature porcine PD-L1 consists of an extracellular domain (ECD) with both an Ig-V and Ig-C-like motif, a single transmembrane domain, and a short cytoplasmic tail lacking a canonical signaling motif (1). The mature ECD of porcine PD-L1 shares 74% and 67% amino acid sequence identity with human and mouse B7-H1, respectively. Alternative splicing of PD-L1 generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (2). B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, endothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma (3-9). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7-9). It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells (6, 10). It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development (9). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells (5, 10). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (3-4, 7, 9).

Item	Value
References	Sun, C. et al. (2018) Immunity. 48:434. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915. Kuang, D.M. et al. (2014) J. Clin. Invest. 124:4657. Cao, Y. et al. (2010) Cancer Res. 71:1235. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625. Dong, H. et al. (2002) Nat. Med. 8:793. Azuma, T. et al. (2008) Blood 111:3635. Park, J.J. et al. (2010) Blood 116:1291. Ritprajak, P. et al. (2010) J. Immunol. 184:4918. Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name	Programmed Death Ligand 1
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names	Avelumab, B7-H, B7-H1, B7H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PD-L1, PD-L1B7 homolog 1, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PDL1PDCD1 ligand 1, Programmed death ligand 1, programmed cell death 1 ligand 1

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