Recombinant Mouse TIM-3 Fc Chimera Protein, CF

Name: Recombinant Mouse TIM-3 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1529-TM
Price: 375 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1529-TM

Citations (2)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	AAL65156
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Mouse TIM-3 Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse TIM-3 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse TIM-3 protein

Mouse TIM-3 (Leu22-Arg191) Accession # AAL65156	PIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu22 & Asp24

Predicted Molecular Mass

45.6 kDa (monomer)

SDS-PAGE

67-80 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin‑9 (Catalog # 2045-GA) at 500 ng/mL can bind recombinant mouse TIM-3 Fc Chimera with an apparent K_D <10 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1529-TM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature mouse TIM-3 consists of a 174 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic tail (3). Within the ECD, mouse TIM-3 shares 58% and 75% aa sequence identity with human and rat TIM-3, respectively. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8⁺, NK, Treg) (4‑11). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (12). TIM-3 ligation attenuates CD8⁺ and Th1 cell responses (12-14) and promotes the activity of Treg and myeloid derived suppressor cells (9, 12, 14, 15). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross‑presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (5). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (11).

Item	Value
References	Sakuishi, K. et al. (2011) Trends Immunol. 32:345. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109. Nakayama, M. et al. (2009) Blood 113:3821. Anderson, A.C. et al. (2007) Science 318:1141. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906. Chiba, S. et al. (2012) Nat. Immunol. 13:832. Monney, L. et al. (2002) Nature 415:536. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093. Ndhlovu, L.C. et al. (2012) Blood 119:3734. Gleason, M.K. et al. (2012) Blood 119:3064. Zhu, C. et al. (2005) Nat. Immunol. 6:1245. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.
Long Name	T Cell Immunoglobulin Mucin-3
Entrez Gene IDs	84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)
Alternate Names	CD366, FLJ14428, HAVCR2, HAVcr-2, KIM-3, SPTCL, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain-containing protein 3, TIM 3, TIM-3, TIM3, TIM3 T-cell membrane protein 3, TIMD-3, TIMD3, TIMD3KIM-3, hepatitis A virus cellular receptor 2, kidney injury molecule-3

Citations for Recombinant Mouse TIM-3 Fc Chimera Protein, CF (2)

Showing 1 - 2 of 2 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TIM-3 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Protein
Applications: Enzyme Assay

Matsumura et al. (2015), Botulinum toxin A complex exploits intestinal M cells to enter the host and exert neurotoxicity. Nat Commun, 6(0):6255.
PMID: 25687350
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Leitner et al. (2013), TIM-3 does not act as a receptor for galectin-9. PLoS Pathog, 9(3):e1003253.
PMID: 23555261

There are no citations that match your criteria.

Showing 1 - 2 of 2 citations Showing All

Product Documents for Recombinant Mouse TIM-3 Fc Chimera Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Mouse TIM-3 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased