Recombinant Mouse FGF-21 Protein

Name: Recombinant Mouse FGF-21 Protein
Brand: R&D Systems
SKU: 8409-FG

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8409-FG

Citations (4)

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Key Product Details

Source:	NS0
Accession #:	Q9JJN1
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse FGF-21 Protein

Source	Mouse myeloma cell line, NS0-derived mouse FGF-21 protein Tyr30-Ser210
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Tyr30 & Tyr49
Predicted Molecular Mass	20 kDa
SDS-PAGE	22-25 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.4-2 μg/mL in the presence of Recombinant Mouse Klotho beta (Catalog # 2619-KB).

Scientific Data Examples for Recombinant Mouse FGF-21 Protein

	FGF-21 Stimulates Cell Proliferation. Recombinant Mouse FGF-21 stimulated proliferation in NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.4-2 µg/mL in the presence of Recombinant Mouse Klotho beta (Catalog # 2619-KB).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

8409-FG

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8409-FG/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-21

Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family. Based on its structure, FGF-21 is further classified into a subfamily of FGFs along with FGF-19 and -23 (1). Mouse FGF-21 is a 210 amino acid (aa) polypeptide that contains a 120 aa core FGF domain and a hydrophobic N-terminus signal sequence. The signal sequence is cleaved to release the soluble 180 aa mature FGF-21 protein (2). At the amino acid sequence level, mature mouse FGF-21 is 81% and 92% identical to mature human and rat FGF-21, respectively. In comparison to other FGF subfamilies, a heparin-binding domain is uniquely absent in FGF-19 subfamily members. Lack of this domain confers endocrine function to FGF-19 members and enables them to freely diffuse within tissues and accumulate in the circulatory system (3, 4). The biological activity of FGF-21 requires binding to Klotho beta, a co-receptor that is in complex with cell surface FGF receptors (FGF R) (5, 6). Binding of FGF-21 to Klotho beta facilitates FGF R activation and autophosphorylation resulting in the initiation of multiple downstream signaling cascades (7, 8). FGF-21 cannot independently bind to FGF Rs, thus its effects are restricted to target tissues that express Klotho beta. FGF-21 functions as a physiological regulator of cellular metabolism, including glucose uptake in adipocytes and cellular sensitivity to insulin. FGF-21 is basally expressed in the pancreas, thymus, and liver, as well as in adipose tissue (3, 9). Local and systemic metabolic stress has been shown to induce expression of FGF-21 in the liver, muscles, and fat (10-12). Modulation of FGF-21 expression is associated with a number of metabolic disorders, including obesity and diabetes (7, 13). FGF-21 is also involved in promoting cell survival and proliferation, modulating mesenchymal stem cell differentiation, regulating circadian rhythm, and controlling reproductive capacity during nutrient deprivation (14-18).

Item	Value
References	Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203. Adams, A.C. et al. (2013) Mol. Metab. 2:205. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417. Suzuki, M. et al. (2008) Mol. Endocrinol. 22:1006. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687. Gimeno, R.E. and D.E. Moller (2014) Trends Endocrinol. Metab. 25:303. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. U S A 104:7432. Kharitonenkov, A. et al. (2005) J. Clin. Invest. 115:1627. Luo, Y. and W.L. McKeehan (2013) Front. Endocrinol. (Lausanne) 4:194. Yang, C. et al. (2013) BMC Gastroenterol. 13:67. Keipert, S. et al. (2014) Am. J. Physiol. Endocrinol. Metab. 306:E469. Zhang, X. et al. (2008) Diabetes 57:1246. Wei, W. et al. (2012) Proc. Natl. Acad. Sci. USA 109:3143. Wei, W. et al. (2010) Cell. Metab. 11:503. Wan, Y. (2013) Int. J. Biochem. Cell. Biol. 45:546. Bookout, A.L. et al. (2013) Nat. Med. 19:1147. Owen, B.M. et al. (2013) Nat. Med. 19:1153.
Long Name	Fibroblast Growth Factor 21
Entrez Gene IDs	26291 (Human); 56636 (Mouse)
Alternate Names	FGF-21, FGF21, fibroblast growth factor 21

Citations for Recombinant Mouse FGF-21 Protein (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse FGF-21 Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

CB Higgins et al. (2022), SIRT1 selectively exerts the metabolic protective effects of hepatocyte nicotinamide phosphoribosyltransferase Nature Communications, 13(1):1074.
PMID: 35228549
Species: Human

Applications: Bioassay

T Kroon et al. (2020), PPAR&gamma and PPAR&alpha synergize to induce robust browning of white fat inï¿½vivo Mol Metab, 36(0):100964.
PMID: 32248079
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Kuroda et al. (2017), Peripherally derived FGF21 promotes remyelination in the central nervous system J. Clin. Invest., 0(0).
PMID: 28825598
Species: Mouse
Sample Types: Serum
Applications: ELISA (Standard)

(2016), Chronic Over-expression of Fibroblast Growth Factor 21 Increases Bile Acid Biosynthesis by Opposing FGF15/19 Action EBioMedicine, 0(0).
PMID: 28041926

Recombinant Mouse FGF-21 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse FGF-21 Protein

Scientific Data Examples for Recombinant Mouse FGF-21 Protein

Formulation, Preparation and Storage

Carrier Free

8409-FG

Carrier

8409-FG/CF

Carrier Free

Reconstitution Calculator

Background: FGF-21

Citations for Recombinant Mouse FGF-21 Protein (4)

Product Documents for Recombinant Mouse FGF-21 Protein

Product Datasheets

COA

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