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Recombinant Mouse Klotho beta Protein, CF

Catalog # 2619-KB | R&D Systems, Inc. a Bio-Techne Brand
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2619-KB-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Klotho beta protein
Phe53-Leu995, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe53

Predicted Molecular Mass

110.2 kDa

SDS-PAGE

140 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc.
The ED50 for this effect is 0.2-1 µg/mL in the presence of Recombinant Human FGF-21 (Catalog # 2539-FG) and Heparin.

Scientific Data Images for Recombinant Mouse Klotho beta Protein, CF

Recombinant Mouse Klotho beta Protein Bioactivity

Recombinant Mouse Klotho beta Protein Bioactivity

Recombinant Mouse Klotho beta (Catalog # 2619-KB) stimulates cell proliferation of the BaF3 mouse pro-B cell line transfected with human FGF RIIIc. The ED50 for this effect is 0.2-1 µg/mL in the presence of Recombinant Human FGF-21 (Catalog # 2539-FG) and Heparin.
Recombinant Mouse Klotho beta Protein SDS-PAGE

Recombinant Mouse Klotho beta Protein SDS-PAGE

1 μg/lane of Recombinant Mouse Klotho beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 135 kDa.

Formulation, Preparation and Storage

2619-KB
Formulation Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Background: Klotho beta

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Like Klotho, Klotho beta facilitates binding between FGF-19 subfamily members and their receptors via formation of a ternary complex (2). The Klotho beta mediated interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2-4). Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes, which allows FGF-21 to stimulate GLUT1 expression, up-regulating adipocyte insulin-dependent glucose uptake (2-3, 5). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta-glycosidases, it was initially unclear whether they were functional enzymes (1, 6). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho  beta is also possible (7). The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.

References

  1. Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
  2. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
  3. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  4. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
  5. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
  6. Chang, Q. et al. (2005) Science 310:490.
  7. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.

Alternate Names

KLB

Entrez Gene IDs

152831 (Human); 83379 (Mouse); 289625 (Rat); 102147155 (Cynomolgus Monkey)

Gene Symbol

KLB

UniProt

Product Documents for Recombinant Mouse Klotho beta Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Klotho beta Protein, CF

For research use only

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