Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain)

Name: Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain)
Brand: R&D Systems
SKU: 458-MF
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 458-MF

Citations (8)

(1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	O35188
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain)

Product Specifications for Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain)

Source	E. coli-derived mouse CX3CL1/Fractalkine protein Leu22-Lys105
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu22
Predicted Molecular Mass	9.5 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED₅₀ for this effect is 10-50 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

458-MF

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

458-MF/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CX3CL1/Fractalkine

Fractalkine, designated CX3CL1 and also known as neurotactin, is the only member of the CX3C, or delta, chemokine subfamily (1 ‑ 4). Unlike most other chemokines, CX3CL1 is a type I transmembrane (TM) adhesion protein (1). The mouse CX3CL1 cDNA encodes 395 amino acids (aa), including a signal sequence (aa 1 ‑ 24), a chemokine domain (aa 25 ‑ 100), a mucin stalk region (aa 101 ‑ 336), a transmembrane segment (aa 337 ‑ 357), and a cytoplasmic tail (aa 358 ‑ 395). The chemokine domain contains binding and chemotactic determinants, while the mucin stalk appears to function only as a spacer (4, 5). Mouse CX3CL1 shares 85% and 78% aa sequence identity with rat and human CX3CL1, respectively, within the chemokine domain, but lower sequence identity within other domains. CX3CL1 is up‑regulated by pro‑inflammatory stimuli, especially IFN‑ gamma and TNF‑ alpha, on cell types including macrophages, dendritic cells, endothelium, neurons, smooth muscle and epithelium lining the intestines and other tubules (1, 8, 9). The 40 kDa, 7‑TM non‑glycosylated G‑protein coupled CX3CL1 receptor, CX3CR1, is expressed by cytotoxic effector cells and cytokine producers, including type I helper and cytotoxic T cells, gamma δ T cells, CD16+ NK cells, monocytes and microglia (1, 2). The 95 ‑ 100 kDa TM CX3CL1 can be inducibly cleaved near the TM segment by ADAM10 or ADAM17 to generate a 60 ‑ 80 kDa soluble form (6, 7). TM CX3CL1 functions as an adhesion molecule, while both forms are chemoattractants for target cells expressing CX3CR1 (1, 2). During extravasation, membrane‑bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis (6). In coronary artery disease, soluble CX3CL1 and CD8+ T cell CX3CR1 are overexpressed and appear to contribute to pathogenesis (1, 10). In the brain, CX3CL1/CX3CR1 interaction protects against microglial neurotoxicity (11). CX3CL1 also contributes to wound healing by recruiting macrophages, and to bone resorption by recruiting and mediating adhesion of osteoclast precursors (12, 13).

Item	Value
References	Stievano, L. et al. (2004) Crit. Rev. Immunol. 24:205. Umehara, H. et al. (2004) Arterioscler. Thromb. Vasc. Biol. 24:34. Rossi, D.L. et al. (1998) Genomics 47:163. Mizoue, L.S. et al. (2001) J. Biol. Chem. 276:33906. Harrison, J.K. et al. (2001) J. Biol. Chem. 276:21632. Hundhausen, C. et al. (2007) J. Immunol. 178:8064. Tsou, C. et al. (2001) J. Biol. Chem. 276:44622. Tarozzo, G. et al. (2003) J. Neurosci. Res. 73:81. Lucas, A.D. et al. (2001) Am. J. Pathol. 158:855. Damas, J.K. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:2567. Cardona, A.E. et al. (2006) Nat. Neurosci. 9:917. Koizumi, K. et al. (2009) J. Immunol. 183:7825. Ishida, Y. et al. (2008) J. Immunol. 180:569.
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names	ABCD-3, C3Xkine, CX3C membrane-anchored chemokine, CX3CL1, CXC3, CXC3C, FKN, Fractalkine, NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, Neurotactin, SCYD1C-X3-C motif chemokine 1, chemokine (C-X3-C motif) ligand 1, neurotactin), small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain) (8)

Showing 1 - 8 of 8 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain) include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Banerjee et al. (2022), CX3CL1 Action on Microglia Protects from Diet-Induced Obesity by Restoring POMC Neuronal Excitability and Melanocortin System Activity Impaired by High-Fat Diet Feeding International Journal of Molecular Sciences, 23(12).
PMID: 35742824
Species: Rat
Sample Types: In Vivo
Applications: Bioassay

ME Martinez et al. (2021), Immunogenicity and inflammatory properties of respiratory syncytial virus attachment G protein in cotton rats PLoS ONE, 16(2):e0246770.
PMID: 33600439
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Yang et al. (2020), A novel role of FKN/CX3CR1 in promoting osteogenic transformation of VSMCs and atherosclerotic calcification Cell Calcium, 91(0):102265.
PMID: 32814243
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

A Voronova et al. (2017), Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain Neuron, 94(3):500-516.e9.
PMID: 28472653
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

SA O'Sullivan et al. (2017), The chemokine fractalkine (CX3CL1) attenuates H2O2-induced demyelination in cerebellar slices J Neuroinflammation, 14(1):159.
PMID: 28810923
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Fuller AD et al. (2008), MFG-E8 regulates microglial phagocytosis of apoptotic neurons. J Neuroimmune Pharmacol, 3(4):246-56.
PMID: 18670887
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Yang XP et al. (2007), Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway. Circ. Res., 101(10):1001-8.
PMID: 17885215
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ueno T et al. (2005), The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 105(1):31-9.
PMID: 15358618

There are no citations that match your criteria.

Showing 1 - 8 of 8 citations Showing All

Customer Reviews for Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain) (1)

4 out of 5

1 customer rating

5 stars

4 stars

100%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain)

Name: Anonymous

Application: Immunoassay Standard

Reason for Rating: It works well but shows a very high reading on mouse ELISA

Verified Customer | Posted 12/05/2016

Compared to FKN in mouse ELISA (DY472)

Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain)

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain)

Formulation, Preparation and Storage

Carrier Free

458-MF

Carrier

458-MF/CF

Carrier Free

Reconstitution Calculator

Background: CX3CL1/Fractalkine

Citations for Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain) (8)

Customer Reviews for Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain) (1)

Customer Images

Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain)

Product Documents for Recombinant Mouse CX3CL1/Fractalkine (Chemokine Domain)

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected