Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Name: Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10140-T2

Catalog # 10140-T2

Citations (1)

Product Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	AAH01281.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TRAIL R2/TNFRSF10B protein

Human TRAIL R2/TNFRSF10B (Ala54-Ser210) Accession # AAH01281.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala54

Predicted Molecular Mass

44 kDa

SDS-PAGE

54-61 kDa, under reducing conditions

Activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 0.75-6 ng/mL.

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein Inhibition Activity Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 10140-T2) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC) mediated cytotoxicity using L-929 mouse fibroblast cells. The ED₅₀ for this effect is 0.75-6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10140-T2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand) (1). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. There are no known rat or mouse homologs to TRAILR2. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis (1-2). The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis (1-2). Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported (3).

Item	Value
References	Chaudhary, P.M. et al. (1997) Immunity 7:821. Walczak, H. et al. (1997) EMBO J. 16:5386. Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name	TRAIL Receptor 2
Entrez Gene IDs	8795 (Human); 21933 (Mouse)
Alternate Names	CD262, CD262 antigen, DR5, DR5TRICK2B, Death receptor 5, Fas-like protein, KILLER/DR5, KILLERapoptosis inducing protein TRICK2A/2B, TNF-related apoptosis-inducing ligand receptor 2, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAIL-R2apoptosis inducing receptor TRAIL-R2, TRAILR2, TRAILR2cytotoxic TRAIL receptor-2, TRICK2, TRICK2A, TRICKB, ZTNFR9, death domain containing receptor for TRAIL/Apo-2L, tumor necrosis factor receptor superfamily member 10B, tumor necrosis factor receptor superfamily, member 10b, tumor necrosis factor receptor-like protein ZTNFR9

Citations for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF include:

Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

S Natesampil et al. (2019), TRAILshort Protects against CD4 T Cell Death during Acute HIV Infection J. Immunol., 0(0).
PMID: 31189571

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