Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Name: Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10140-T2
Price: 405 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10140-T2

Citations (3)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	AAH01281.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Product Specifications for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human TRAIL R2/TNFRSF10B protein

Human TRAIL R2/TNFRSF10B (Ala54-Ser210) Accession # AAH01281.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala54

Predicted Molecular Mass

44 kDa

SDS-PAGE

54-61 kDa, under reducing conditions

Activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 0.75-6 ng/mL.

Scientific Data Examples for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

	Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein Inhibition Activity Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 10140-T2) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC) mediated cytotoxicity using L-929 mouse fibroblast cells. The ED₅₀ for this effect is 0.75-6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10140-T2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand) (1). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. There are no known rat or mouse homologs to TRAILR2. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis (1-2). The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis (1-2). Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported (3).

Item	Value
References	Chaudhary, P.M. et al. (1997) Immunity 7:821. Walczak, H. et al. (1997) EMBO J. 16:5386. Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name	TRAIL Receptor 2
Entrez Gene IDs	8795 (Human); 21933 (Mouse)
Alternate Names	CD262, CD262 antigen, DR5, DR5TRICK2B, Death receptor 5, Fas-like protein, KILLER/DR5, KILLERapoptosis inducing protein TRICK2A/2B, TNF-related apoptosis-inducing ligand receptor 2, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAIL-R2apoptosis inducing receptor TRAIL-R2, TRAILR2, TRAILR2cytotoxic TRAIL receptor-2, TRICK2, TRICK2A, TRICKB, ZTNFR9, death domain containing receptor for TRAIL/Apo-2L, tumor necrosis factor receptor superfamily member 10B, tumor necrosis factor receptor superfamily, member 10b, tumor necrosis factor receptor-like protein ZTNFR9

Citations for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cell
Applications: Cell Culture

MW Lee et al. (2022-01-01), Dual role of ERK2/NF-kappaB signaling in TRAIL sensitivity American journal of cancer research, 12(7):3373-3389.
PMID: 35968322
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture

AV Yagolovich et al. (2022-01-01), DR5-Selective TRAIL Variant DR5-B Functionalized with Tumor-Penetrating iRGD Peptide for Enhanced Antitumor Activity against Glioblastoma International Journal of Molecular Sciences, 23(20).
PMID: 36293545
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

S Natesampil et al. (2019-01-01), TRAILshort Protects against CD4 T Cell Death during Acute HIV Infection J. Immunol., 0(0).
PMID: 31189571

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: TRAIL R2/TNFRSF10B

Citations for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF (3)

Product Documents for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected