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Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Bio-Techne includes R&D Systems | Catalog # 375-TEC

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Carrier Free
375-TEC-010/CF

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With Carrier
375-TEC-010

Key Product Details

Source

E. coli

Accession #

Structure / Form

Homotrimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human TRAIL/TNFSF10 protein
Val114-Gly281, with and without an N-terminal Met

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val114

Predicted Molecular Mass

19 kDa (monomer)

Activity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.
The ED50 for this effect is 0.5-6 ng/mL.
Note: This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the variants.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 375-TEC
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, (NH4)2SO4 and ZnSO4 with BSA as a carrier protein.
Reconstitution Reconstitute at 20 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 1 month, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 375-TEC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, (NH4)2SO4 and ZnSO4.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 1 month, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL/TNFSF10

TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is the most homologous to Fas ligand, sharing 28% aa sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% aa sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that transduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1 - 3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4). It was shown that homotrimeric TRAIL binds a zinc ion which is critical for the correct structure of the protein (5 - 6).

References

  1. Golstein, P. (1997) Current Biology 7:R750.
  2. Wiley, S.R. et al. (1995) Immunity 3:673.
  3. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
  4. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.
  5. Bodmer, J.L. et al. (2000) J. Biol. Chem. 275:20632.
  6. Hymowitz, S.G. et al. (2000) Biochemistry 39:633.

Long Name

TNF-related Apoptosis-inducing Ligand

Alternate Names

CD253, TNFSF10

Entrez Gene IDs

8743 (Human); 22035 (Mouse); 246775 (Rat)

Gene Symbol

TNFSF10

UniProt

Additional TRAIL/TNFSF10 Products

Product Documents for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TRAIL/TNFSF10 (E. coli-expressed) Protein

For research use only

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