Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

Name: Recombinant Human TIGIT (A103) Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 7898-TGB
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7898-TGB

Citations (1)

(2)

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Key Product Details

Source:	CHO
Accession #:	AAI01289
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

Product Specifications for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human TIGIT protein

Human TIGIT (Met22 - Pro141, A103) Accession # AAI01289	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met22

Predicted Molecular Mass

40 kDa

SDS-PAGE

40-53 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR (Catalog # 2530-CD) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human TIGIT (A103) Fc Chimera that produces 50% optimal binding response is 40-200 ng/mL.

Scientific Data Examples for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

	Recombinant Human TIGIT (A103) Fc Chimera Protein Binding Activity When Recombinant Human CD155/PVR (Catalog # 2530-CD) is immobilized at 2.5 µg/mL, Recombinant Human TIGIT (A103) Fc Chimera (Catalog # 7898-TGB) binds with an ED₅₀ of 40-200 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7898-TGB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).

Item	Value
References	Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695. Yu, X. et al. (2009) Nat. Immunol. 10:48. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11. SwissProt Accession # Q495A1. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858. Joller, N. et al. (2011) J. Immunol. 83:1338.
Long Name	T Cell Immunoreceptor with Ig and ITIM Domains
Entrez Gene IDs	201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)
Alternate Names	T cell immunoreceptor with Ig and ITIM domains, TIGIT, VSIG9, VSTM3, WUCAM

Citations for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wang F et al. (2015), TIGIT expression levels on human NK cells correlate with functional heterogeneity among healthy individuals. Eur J Immunol, 45(10):2886-97.
PMID: 26171588

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Customer Reviews for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF (2)

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Recombinant Human TIGIT Fc Chimera Protein, CF

Name: Vijay Patel

Application: Immunoassay Standard

Verified Customer | Posted 12/10/2020
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Name: Anonymous

Verified Customer | Posted 07/19/2020

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Product Documents for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

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