Recombinant Human Sonic Hedgehog/Shh Protein, High Activity Best Seller

Name: Recombinant Human Sonic Hedgehog/Shh Protein, High Activity
Brand: R&D Systems
SKU: 8908-SH
Rating: 4 (1 reviews)

Catalog # 8908-SH | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (9)

Product Datasheet / COA / SDS

C-terminal cholesterol, N-terminal fatty acid-modified

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	Q15465
Structure / Form:	Cholesterol-modified at the C-terminal and fatty acid-modified at the N-terminal
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

Product Specifications for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

Source	Human embryonic kidney cell, HEK293-derived human Sonic Hedgehog/Shh protein Cys24-Gly197
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Cys24
Predicted Molecular Mass	20 kDa
SDS-PAGE	18-24 kDa, reducing conditions
Activity	Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED₅₀ for this effect is typically 6-36 ng/mL.

Scientific Data Examples for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

	Enhanced Activity of Human Cell-expressed Sonic Hedgehog (Shh) Protein. Recombinant Human Shh proteins induce alkaline phosphatase production by mesenchymal stem cells. High Activity Shh (green), purified from HEK293 cells and containing the correct post-translational modifications (cholesterol and fatty acids), is over 14-fold more active than E. coli-purified Recombinant Human Shh-N (C24II) N-Terminus (Catalog # 1845-SH; red line), and over 250-fold more active than E. coli-purified Recombinant Human Shh-N (Catalog # 1314-SH; blue line).
	Enhanced Activity of Human Cell-expressed Sonic Hedgehog (Shh) Protein. 1 μg/lane of Recombinant Human Sonic Hedgehog/Shh Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing 18-24 kDa bands.
	Post-translational Modification Analysis of Naturally-modified Recombinant Human Sonic Hedgehog (Shh) Protein. LC/ESI-MS analysis of Recombinant Human (rh)SHH Protein, High Activity shows major peaks at 20119.3, 20145.2, and 20171.6 Da, suggesting that recombinant human SHH molecules are dual-modified with cholesterol at C-terminus, and fatty acids (lauric acid, myristic acid, and palmitic acid) at the N-terminus. The minor peaks at 19776 Da corresponds to rhSHH with only fatty acid modification.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

8908-SH

Carrier

Formulation	Supplied as a 0.2 μm filtered solution in MES, NaCl and CHAPS with BSA as a carrier protein.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

8908-SH/CF

Carrier Free

Formulation	Supplied as a 0.2 μm filtered solution in MES, NaCl and CHAPS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Sonic Hedgehog/Shh

The Sonic Hedgehog (Shh) protein is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. The Shh protein regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue, Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor Shh protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). The Shh-C protein, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). The Shh-N protein is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of the Shh protein as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase the receptor binding affinity and signaling potency of the Shh-N protein (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of functionality of the Shh protein and are required for the development of Shh protein gradients important in tissue morphogenesis (9, 13). Canonical signaling by the Shh protein is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). Binding of the Shh protein to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

Item	Value
References	Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86. Xie, J. et al. (2013) OncoTargets Ther. 6:1425. Marigo, V. et al. (1995) Genomics 28:44. Zeng, X. et al. (2001) Nature 411:716. Feng, J. et al. (2004) Development 131:4357. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037. Chen, M.-H. et al. (2004) Genes Dev. 18:641. Etheridge, L.A. et al. (2010) Development 137:133. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013. Lewis, P.M. et al. (2001) Cell 105:599. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
Entrez Gene IDs	6469 (Human); 20423 (Mouse)
Alternate Names	HHG-1, HHG1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), Shh, ShhNC, Sonic Hedgehog, TPT, TPTPS, sonic hedgehog homolog, sonic hedgehog protein

Citations for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity (9)

Showing 1 - 9 of 9 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity include:

Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

W Li et al. (2021-11-29), Structural insights into proteolytic activation of the human Dispatched1 transporter for Hedgehog morphogen release Nature Communications, 2021-112(1):6966.
PMID: 34845226
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay

FJ Gao et al. (2021-08-16), Forebrain Shh overexpression improves cognitive function and locomotor hyperactivity in an aneuploid mouse model of Down syndrome and its euploid littermates Acta neuropathologica communications, 2021-09(1):137.
PMID: 34399854
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Sun et al. (2021-07-29), SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts Nature Communications, 2021-012(1):4611.
PMID: 34326333
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Xu et al. (2021-02-26), PAUPAR and PAX6 sequentially regulate human embryonic stem cell cortical differentiation Nucleic Acids Research, 2021-00(0).
PMID: 33544864
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Ichimiya et al. (2021-01-22), GLI2 but not GLI1/GLI3 plays a central role in the induction of malignant phenotype of gallbladder cancer Oncology reports, 2021-045(3):997-1010.
PMID: 33650666
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SM Griffin et al. (2020-09-14), Nicotinamide restricts neural precursor proliferation to enhance catecholaminergic neuronal subtype differentiation from mouse embryonic stem cells PLoS ONE, 2020-015(9):e0233477.
PMID: 32925933
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JW Kim et al. (2020-08-25), Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis Cell Stem Cell, 2020-027(4):633-645.e7.
PMID: 32846140
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

X Qi et al. (2018-07-11), Structures of human Patched and its complex with native palmitoylated sonic hedgehog Nature, 2018-00(0).
PMID: 29995851
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

N Kurio et al. (2018-02-12), Roles of Ihh signaling in chondroprogenitor function in postnatal condylar cartilage Matrix Biol., 2018-00(0).
PMID: 29447948

There are no citations that match your criteria.

Showing 1 - 9 of 9 citations Showing All

Customer Reviews for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity (1)

4 out of 5

1 customer rating

5 stars

4 stars

100%

3 stars

2 stars

1 star

Showing 1 - 1 of 1 review Showing All

Filter By:

Name: Boubacar Barry

Application: Binding assay/Protein-protein interaction

Reason for Rating: n/a

Verified Customer | Posted 08/11/2017

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

Recombinant Human Sonic Hedgehog/Shh Protein, High Activity Best Seller

Catalog # 8908-SH | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

Scientific Data Examples for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

Formulation, Preparation and Storage

Carrier Free

8908-SH

Carrier

8908-SH/CF

Carrier Free

Reconstitution Calculator

Background: Sonic Hedgehog/Shh

Citations for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity (9)

Customer Reviews for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity (1)

Product Documents for Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected