What is the difference between Recombinant Human Sonic Hedgehog Catalog # 1845-SH and Catalog # 1314-SH?

Name: Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein
Brand: R&D Systems
SKU: 1314-SH
Price: 293 EUR

Recombinant Human Sonic Hedgehog, Catalog # 1845-SH, possesses a N-terminal mutation that increases its potency in bioassay tests. The amino acid sequence is Cys24-Gly197 (Cys24Ile-Ile), accession number NP_000184. The Cys24Ile-Ile mutation was created to match a publication that describes enhanced activity with these modifications: "http://www.ncbi.nlm.nih.gov/pubmed/11284692". Catalog # 1314-SH does not possess this mutation.

Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

Catalog # 1314-SH | R&D Systems, Inc. a Bio-Techne Brand

Citations (14)

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Key Product Details

Source:	E. coli
Accession #:	Q15465
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

Product Specifications for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

Source	E. coli-derived human Sonic Hedgehog/Shh protein Cys24-Gly197, with a C-terminal 6-His tag
Purity	>97%, by SDS-PAGE with silver staining, under reducing conditions
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Cys24
Predicted Molecular Mass	20 kDa
SDS-PAGE	22 kDa, reducing conditions
Activity	Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED₅₀ for this effect is <5 µg/mL.

Scientific Data Examples for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

	Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein Bioactivity Recombinant Human Sonic Hedgehog/Shh, N-Terminus (Catalog # 1314-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED₅₀ for this effect is <5 μg/mL.
	Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein SDS-PAGE 1 μg/lane of Recombinant Human Sonic Hedgehog/Shh, N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1314-SH

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄, NaCl and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1314-SH/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄, NaCl and DTT.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

Item	Value
References	Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86. Xie, J. et al. (2013) OncoTargets Ther. 6:1425. Marigo, V. et al. (1995) Genomics 28:44. Zeng, X. et al. (2001) Nature 411:716. Feng, J. et al. (2004) Development 131:4357. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037. Chen, M.-H. et al. (2004) Genes Dev. 18:641. Etheridge, L.A. et al. (2010) Development 137:133. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013. Lewis, P.M. et al. (2001) Cell 105:599. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
Entrez Gene IDs	6469 (Human); 20423 (Mouse)
Alternate Names	HHG-1, HHG1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), Shh, ShhNC, Sonic Hedgehog, TPT, TPTPS, sonic hedgehog homolog, sonic hedgehog protein

Citations for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein (14)

Showing 1 - 10 of 14 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein include:

Species: Human
Sample Types: Cell Lysates
Applications: ELISA Capture

J Sun et al. (2021-07-29), SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts Nature Communications, 2021-012(1):4611.
PMID: 34326333
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Kim et al. (2020-04-24), Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration Nat Commun, 2020-011(1):1994.
PMID: 32332736
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

Y Arribat et al. (2019-12-02), Sonic Hedgehog repression underlies gigaxonin mutation-induced motor deficits in giant axonal neuropathy J. Clin. Invest., 2019-10(0).
PMID: 31503551
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Johansen et al. (2017-10-22), Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells Stem Cells Int, 2017-12017(0):9605432.
PMID: 29201062
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

E El Agha et al. (2017-06-01), Ex vivo analysis of the contribution of FGF10(+) cells to airway smooth muscle cell formation during early lung development Dev. Dyn., 2017-00(0).
PMID: 28387977
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Dye et al. (2015-03-24), In vitro generation of human pluripotent stem cell derived lung organoids. Elife, 2015-04(0).
PMID: 25803487
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Xu Y et al. (2013-11-28), Inhibition of the Hedgehog pathway induces autophagy in pancreatic ductal adenocarcinoma cells. Oncol Rep, 2013-131(2):707-12.
PMID: 24297612
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Bioassay

Mills et al. (2013-03-12), Loss of the transcription factor GLI1 identifies a signaling network in the tumor microenvironment mediating KRAS oncogene-induced transformation. J Biol Chem, 2013-0288(17):11786-94.
PMID: 23482563
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

Aad et al. (2012-10-04), The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems. Biol Reprod, 2012-187(4):79.
PMID: 22811575
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Erceg S et al. (2012-03-01), Derivation of cerebellar neurons from human pluripotent stem cells. Curr Protoc Stem Cell Biol, 2012-020(0):1H.51.
PMID: 22415839
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gil JE et al. (2009-01-20), Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett., 2009-0583(3):561-7.
PMID: 19162023
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Elkabetz Y et al. (2008-01-15), Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes Dev., 2008-022(2):152-65.
PMID: 18198334
Sample Types: N/A
Applications: SDS-Page

Zavros Y et al. (2007-09-14), Reduced pepsin A processing of sonic hedgehog in parietal cells precedes gastric atrophy and transformation. J. Biol. Chem., 2007-0282(46):33265-74.
PMID: 17872943
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay

Nordstrom U et al. (2006-07-01), An early role for WNT signaling in specifying neural patterns of Cdx and Hox gene expression and motor neuron subtype identity. PLoS Biol., 2006-04(8):e252.
PMID: 16895440

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Showing 1 - 10 of 14 citations Showing All

FAQs for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

What is the difference between Recombinant Human Sonic Hedgehog Catalog # 1845-SH and Catalog # 1314-SH?

Recombinant Human Sonic Hedgehog, Catalog # 1845-SH, possesses a N-terminal mutation that increases its potency in bioassay tests. The amino acid sequence is Cys24-Gly197 (Cys24Ile-Ile), accession number NP_000184. The Cys24Ile-Ile mutation was created to match a publication that describes enhanced activity with these modifications: "http://www.ncbi.nlm.nih.gov/pubmed/11284692". Catalog # 1314-SH does not possess this mutation.

Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

Catalog # 1314-SH | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

Scientific Data Examples for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

Formulation, Preparation and Storage

Carrier Free

1314-SH

Carrier

1314-SH/CF

Carrier Free

Reconstitution Calculator

Background: Sonic Hedgehog/Shh

Citations for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein (14)

FAQs for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

Product Documents for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

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