Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

Name: Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein
Brand: R&D Systems
SKU: AFR9049

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AFR9049

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His-tag

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Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	Q9NZQ7.1
Structure / Form:	Labeled with Alexa Fluor® 647 Excitation Wavelength: 650 nm Emission Wavelength: 668 nm
Applications:	Bioactivity

View Full Technical Details for Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

Product Specifications for Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

Source	Human embryonic kidney cell, HEK293-derived human PD-L1/B7-H1 protein Phe19-Thr239, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe19
Predicted Molecular Mass	26 kDa
SDS-PAGE	31-42 kDa, under reducing conditions.
Activity	Measured by flow cytometry for its ability to bind anti-human PD-L1/B7-H1 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human PD-L1/B7-H1 His-tag Alexa Fluor® 647 (Catalog # AFR9049) that produces 50% of the binding response is 0.500‑5.00 ng/mL.

Scientific Data Examples for Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

	Flow cytometry analysis for Recombinant Human PD-L1/B7-H1 His-tag Alexa Fluor® 647 staining on anti-human PD-L1/B7-H1 Monoclonal Antibody conjugated beads. Streptavidin coated beads conjugated to biotinylated anti-human PD-L1/B7-H1 Monoclonal Antibody were stained with the indicated concentrations of Recombinant Human PD-L1/B7-H1 His-tag Alexa Fluor® 647 (Catalog # AFR9049).
	Recombinant Human PD-L1/B7-H1 His-tag Alexa Fluor® 647 Protein SDS-PAGE. 2 μg/lane of Recombinant Human PD-L1/B7-H1 His-tag Alexa Fluor® 647 Protein (Catalog # AFR9049) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 31-42 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

AFR9049

Formulation	Supplied as a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, human B7-H1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (3). B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (4-7), keratinocytes (8, 9), enothelial and intestinal epithelial cells (8, 10), as well as a variety of carcinomas and melanoma (11, 12). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7, 8, 12-15). It suppresses T cell activation and proliferation (5, 8, 14, 16) and induces the apoptosis of activated T cells (11). It plays a role in the development of immune tolerance by promoting T cell anergy (7, 14) and enhancing regulatory T cell development (16). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (5, 10) and inhibits the development of Th17 cells (16). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (6, 9, 12, 15).

Item	Value
References	Ceeraz, S. et al. (2013) Trends Immunol. 34:556. Dong, H. et al. (1999) Nat. Med. 5:1365. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915. Tamura, H. et al. (2001) Blood 97:1809. Chen, L. et al. (2007) J. Immunol. 178:6634. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657. Tsushima, F. et al. (2007) Blood 110:180. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581. Cao, Y. et al. (2010) Cancer Res. 71:1235. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625. Dong, H. et al. (2002) Nat. Med. 8:793. Azuma, T. et al. (2008) Blood 111:3635. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567. Park, J.-J. et al. (2010) Blood 116:1291. Ritprajak, P. et al. (2010) J. Immunol. 184:4918. Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name	Programmed Death Ligand 1
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names	Avelumab, B7-H, B7-H1, B7H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PD-L1, PD-L1B7 homolog 1, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PDL1PDCD1 ligand 1, Programmed death ligand 1, programmed cell death 1 ligand 1

Product Specific Notices for Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

Scientific Data Examples for Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PD-L1/B7-H1

Product Specific Notices for Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

Citations for Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

Product Documents for Recombinant Human PD-L1/B7-H1 His Alexa Fluor® 647 Protein

Product Datasheets

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