Recombinant Human PD-L1/B7-H1 Fc Chimera Biotin Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Recombinant Human PD-L1/B7-H1 Fc Chimera Biotin Protein Binding Activity
(2)
Recombinant Human PD-L1/B7-H1 Fc Chimera Biotin Protein SDS-PAGE
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BT156-050
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Key Product Details

Source: NS0
Accession #: Q9NZQ7
Structure / Form: Disulfide-linked homodimer, biotinylated via amines.
Applications: Bioactivity
  • Formulation, Preparation & Storage

    • Formulation, Preparation and Storage

    Carrier Free
    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    BT156
    Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
    Reconstitution Reconstitute at 500 μg/mL in PBS.
    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
  • FAQs

    • Citations for Recombinant Human PD-L1/B7-H1 Fc Chimera Biotin Protein, CF


    Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

    FAQs for Recombinant Human PD-L1/B7-H1 Fc Chimera Biotin Protein, CF

    • The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

      Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

    • What is the recommended method for reconstitution of a lyophilized protein or antibody?

      Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.   

    • Are R&D Systems recombinant proteins and antibodies sterile?

      Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

    Product Documents for Recombinant Human PD-L1/B7-H1 Fc Chimera Biotin Protein, CF

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Download SDS