Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

Name: Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI1086
Price: 515 USD

Catalog # AVI1086 | R&D Systems, Inc. a Bio-Techne Brand

Product Datasheet / COA / SDS

Biotinylated

Setting the standard in quality research reagents

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source:	CHO
Accession #:	Q15116.3
Structure / Form:	Disulfide-linked homodimer, biotinylated via Avi-tag
Applications:	Bioactivity

View Full Technical Details for Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

Product Specifications for Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human PD-1 protein

Human PD-1
(Leu25-Thr168)
Accession # Q15116.3

IEGRMD

Human IgG₁
(Pro100-Lys330)

HP, 3x (GGGS)

Avi-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu25

Predicted Molecular Mass

45 kDa

SDS-PAGE

62-88 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) that produces 50% of the optimal binding response is 10-90 ng/mL.

Scientific Data Examples for Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

	Recombinant Human PD-1 Fc Chimera Avi-tag Protein Binding Activity When Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) is immobilized at 1 µg/mL, 100 µg/well, Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) binds with an ED₅₀ of 0.01-0.09 µg/mL.
	Recombinant Human PD-1 Fc Chimera Avi-tag Protein SDS-PAGE 2 μg/lane of Biotinylated Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-88 kDa and 120-180 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1086

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature mouse PD-1 consists of a 149 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The mouse PD-1 ECD shares 65% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces
co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13).

Item	Value
References	Ishida, Y. et al. (1992) EMBO J. 11:3887. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265. Watanabe, N et al. (2003) Nat. Immunol. 4:670. Zhang, X. et al. (2004) Immunity 20:337. Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483. Nishimura, H et al. (1996) Int. Immunol. 8:773. Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677. Butte, M.J. et al. (2007) Immunity 27:111. Okazaki, T. et al. (2013) Nat. Immunol. 14:1212. Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293. Nogrady, B. (2014) Nature 513:S10.
Long Name	Programmed Death-1
Entrez Gene IDs	5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names	CD279, CD279 antigen, PD-1, PD1, PD1hPD-l, PDCD1, Protein PD-1, SLEB2, hPD-1, programmed cell death 1, programmed cell death protein 1

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Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

Catalog # AVI1086 | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

Scientific Data Examples for Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PD-1

Citations for Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

Product Documents for Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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