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Recombinant Human Langerin/CD207 Fc Chimera Protein, CF

Catalog # 10401-LN | R&D Systems, Inc. a Bio-Techne Brand
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10401-LN-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Langerin/CD207 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human Langerin/CD207
(Pro65-Pro328)
Accession # Q9UJ71.2
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

56 kDa

SDS-PAGE

58-71 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Langerin/CD207 Fc Chimera (Catalog # 10401-LN) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Mannose-Polyacrylamide binds with an ED50 of 20-160 ng/mL.

Scientific Data Images for Recombinant Human Langerin/CD207 Fc Chimera Protein, CF

Recombinant Human Langerin/CD207 Fc Chimera Protein Binding Activity

Recombinant Human Langerin/CD207 Fc Chimera Protein Binding Activity

When Recombinant Human Langerin/CD207 Fc Chimera (10401-LN) is immobilized at 0.2 μg/mL (100 μL/well), Biotinylated Mannose-Polyacrylamide binds with an ED50 of 20-160 ng/mL.
Recombinant Human Langerin/CD207 Fc Chimera Protein SDS-PAGE

Recombinant Human Langerin/CD207 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human Langerin/CD207 Fc Chimera (Catalog # 10401-LN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-71 kDa and 116-142 kDa, respectively.

Formulation, Preparation and Storage

10401-LN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Langerin/CD207

Langerin (also known as CD207) is a transmembrane glycoprotein within the type II C-Type lectin receptor family and has been identified as a pathogen binding receptor for immune regulation (1). Human langerin consists of an extracellular domain (ECD) containing a coiled-coil domain and a single C-type lectin domain, a transmembrane domain and a short cytoplasmic domain with a proline-rich motif. The mature ECD of human langerin shares 68%, 62%, 71% amino acid identity with mouse, rat and bovine langerin ECD, respectively. Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain "tennis racket"-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1-3). Langerin is necessary and sufficient for Birbeck granule formation (1). Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self-reactions (4-6). Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12).

References

  1. Valladeau, J. et al. (2000) Immunity 12:71.
  2. Valladeau, J. et al. (2003) Immunol. Res. 28:93.
  3. McDermott, R. et al. (2002) Mol. Biol. Cell 13:317.
  4. Stambach, N. S. and M. E. Taylor (2003) Glycobiology 13:401.
  5. Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549.
  6. Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51.
  7. Takahara, K. et al. (2003) Int. Immunol. 16:819.
  8. Hunger, R. E. et al. (2004) J. Clin. Invest. 113:701.
  9. De Witte, L. et al. (2007) Nat. Med. 13:367.
  10. Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
  11. Ward, E. M. et al. (2006) J. Biol. Chem. 281:15450.
  12. Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.

Alternate Names

CD207

Entrez Gene IDs

50489 (Human); 246278 (Mouse)

Gene Symbol

CD207

UniProt

Additional Langerin/CD207 Products

Product Documents for Recombinant Human Langerin/CD207 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Langerin/CD207 Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

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