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Recombinant Human IL-3 Protein Best Seller

Catalog # 203-IL | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Carrier Free
203-IL-010/CF
203-IL-01M/CF
Show More Sizes
203-IL-050/CF
203-IL-100/CF
203-IL-250/CF
203-IL-500/CF
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Catalog #
Availability
Size / Price
Qty
With Carrier
203-IL-010
203-IL-01M
Show More Sizes
203-IL-050
203-IL-100
203-IL-250
203-IL-500
Show Fewer Sizes

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human IL-3 protein
Ala20-Phe152, with and without an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20 and Met

Predicted Molecular Mass

15 kDa

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.02-0.1 ng/mL.

Scientific Data Images for Recombinant Human IL-3 Protein

Recombinant Human IL‑3 Protein SEC-MALS.

Recombinant Human IL-3 Protein (Catalog # 203-IL) has a molecular weight (MW) of 14.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Human IL-3 Protein Bioactivity

Recombinant Human IL-3 Protein Bioactivity

Recombinant Human IL-3 (Catalog # 203-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.02-0.1 ng/mL.
Recombinant Human IL-3 Protein SDS-PAGE

Recombinant Human IL-3 Protein SDS-PAGE

1 μg/lane of Recombinant Human IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 203-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 203-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the  alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Entrez Gene IDs

3562 (Human); 16187 (Mouse); 24495 (Rat)

Gene Symbol

IL3

UniProt

Additional IL-3 Products

Product Documents for Recombinant Human IL-3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-3 Protein

For research use only

Citations for Recombinant Human IL-3 Protein (203)

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Customer Reviews for Recombinant Human IL-3 Protein (5)

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  • Recombinant Human IL-3 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 04/16/2023
    Recombinant Human IL-3 Protein 203-IL
  • Recombinant Human IL-3 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 01/01/2022
    Recombinant Human IL-3 Protein 203-IL
  • Recombinant Human IL-3 Protein
    Name: Meilyn Sylvestre
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 11/20/2020
  • Recombinant Human IL-3 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 10/13/2020
    It's good for hematopoietic colony formation.
    Recombinant Human IL-3 Protein 203-IL
  • Recombinant Human IL-3 Protein
    Name: Zhenping Wang
    Application: Cell Differentiation/Maturation
    Verified Customer | Posted 09/30/2020
    I used this human IL-3 for mast cells differentiation.
    Recombinant Human IL-3 Protein 203-IL

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FAQs for Recombinant Human IL-3 Protein

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  • Q: Can I reconstitute Recombinant Human IL-3 Protein (catalog # 203-IL) at a different concentration than recommended?

    A: We cannot guarantee the performance if the protein is reconstituted at a different concentration than recommended in the product datasheet. Please contact Technical Service if you have any questions or concerns about reconstitution.

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Reconstitution Calculator

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