Recombinant Human Thrombopoietin/TPO Protein, Animal-Free Best Seller

R&D Systems, part of Bio-Techne | Catalog # BT-TPO-AFL

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

NP_000451.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Thrombopoietin/Tpo Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Thrombopoietin/Tpo protein
Ser22-Leu195
Produced using non-animal reagents in an animal-free laboratory.

Purity

>95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala-Ser22

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-19 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 0.050-0.500 ng/mL.
The specific activity of Recombinant Human Thrombopoietin is >1 x 107 units/mg, which is calibrated against the human Thrombopoietin reference standard (NIBSC code: 03/124).

Scientific Data Images for Recombinant Human Thrombopoietin/TPO Protein, Animal-Free

Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human Thrombopoietin/Tpo proteins.

Equivalent bioactivity of GMP (BT-TPO-GMP), Animal-Free (Catalog # BT-TPO-AFL) and RUO (BT-TPO) grades of Recombinant Human Thrombopoietin/Tpo proteins as measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells (orange, green, red, respectively).

Animal-Free™ Recombinant Human Thrombopoietin/Tpo Protein Bioactivity.

Animal-Free™ Recombinant Human Thrombopoietin/Tpo (Catalog # BT-TPO-AFL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.050-0.500 ng/mL.

Animal-Free™ Recombinant Human Thrombopoietin/Tpo Protein SDS-PAGE.

2 μg/lane of Animal-Free™ Recombinant Human Thrombopoietin/Tpo Protein (Catalog # BT-TPO-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-19 kDa, under reducing conditions.

Formulation, Preparation and Storage

BT-TPO-AFL
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin/Tpo

Thrombopoietin (TPO) is a crucial regulator of hematopoietic stem cell (HSC) differentiation, maturation, and proliferation, as well as megakaryocytopoiesis and thrombopoiesis. TPO is often used in conjunction with Stem Cell Factor (SCF) and Flt-3 ligand in cell culture protocols to expand HSCs for bone marrow transplantation and cellular therapies. When combined with SCF and Flt-3 ligand, it promotes the differentiation of HSCs into megakaryocytes, leading to the production of platelets. It has shown promise in emerging cellular therapies for treating sickle cell disease, beta thalassemia, and other blood-related disorders. Moreover, TPO in combination with other cytokines is utilized in the generation of iNK cells, which have the potential to be incorporated into cellular immunotherapies.

Alternate Names

MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Gene Symbol

THPO

UniProt

NP_000451.1

Additional Thrombopoietin/Tpo Products

Product Documents for Recombinant Human Thrombopoietin/TPO Protein, Animal-Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.  

 Production

  • All molecular biology procedures use animal-free media and dedicated labware.
  • Dedicated fermentors are utilized in committed animal-free areas.

Purification

  • Protein purification columns are animal-free.
  • Bulk proteins are filtered using animal-free filters.
  • Purified proteins are stored in animal-free containers in a dedicated cold storage room.

    Quality Assurance

    • Low Endotoxin Level.
    • No impairment of biological activity.
    • High quality product obtained under stringent conditions.
    • For ex vivo research or bioproduction, additional documentation can be provided.

    Please read our complete Animal-Free Statement

    Product Specific Notices for Recombinant Human Thrombopoietin/TPO Protein, Animal-Free

    For research use or further manufacturing only

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