Recombinant Human Thrombopoietin/TPO Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT-TPO

R&D Systems, part of Bio-Techne
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BT-TPO-010
BT-TPO-01M
BT-TPO-050
BT-TPO-100
BT-TPO-250
BT-TPO-500
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Thrombopoietin/TPO Protein (BT-TPO)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

NP_000451.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Thrombopoietin/Tpo Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Thrombopoietin/Tpo protein
Ser22-Leu195

Purity

>95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala-Ser22

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-19 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 0.0500-0.500 ng/mL.

Scientific Data Images for Recombinant Human Thrombopoietin/TPO Protein, CF

Equivalent Bioactivity of GMP,Animal-Free, and RUO gradesof Recombinant HumanThrombopoietin/Tpo proteins.

Equivalent bioactivity of GMP(BT-TPO-GMP),Animal-Free (BT-TPO-AFL) and RUO (Catalog # BT-TPO) grades of RecombinantHuman Thrombopoietin/Tpoproteins as measured in a cellproliferation assay using MO7ehuman megakaryocytic leukemiccells (orange, green, red,respectively).

Recombinant Human Thrombopoietin/Tpo Protein Bioactivity.

Recombinant Human Thrombopoietin/Tpo (Catalog # BT-TPO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.0500-0.500 ng/mL.

Recombinant Human Thrombopoietin/Tpo Protein SDS-PAGE.

2 μg/lane of Recombinant Human Thrombopoietin/Tpo Protein (Catalog # BT-TPO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-19 kDa, under reducing conditions.

Formulation, Preparation and Storage

BT-TPO
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose. 
Reconstitution Reconstitute the 10 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin/Tpo

Thrombopoietin (TPO) is a crucial regulator of hematopoietic stem cell (HSC) differentiation, maturation, and proliferation, as well as megakaryocytopoiesis and thrombopoiesis. TPO is often used in conjunction with Stem Cell Factor (SCF) and Flt-3 ligand in cell culture protocols to expand HSCs for bone marrow transplantation and cellular therapies. When combined with SCF and Flt-3 ligand, it promotes the differentiation of HSCs into megakaryocytes, leading to the production of platelets. It has shown promise in emerging cellular therapies for treating sickle cell disease, beta thalassemia, and other blood-related disorders. Moreover, TPO in combination with other cytokines is utilized in the generation of iNK cells, which have the potential to be incorporated into cellular immunotherapies.

Alternate Names

MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Gene Symbol

THPO

UniProt

NP_000451.1

Additional Thrombopoietin/Tpo Products

Product Documents for Recombinant Human Thrombopoietin/TPO Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Thrombopoietin/TPO Protein, CF

For research use only

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