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Recombinant Human gp130 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11067-GP

R&D Systems, part of Bio-Techne
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11067-GP-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human gp130 protein
Glu23-Ile618, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu23

Predicted Molecular Mass

69 kDa

SDS-PAGE

86-100 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human gp130 His-tag (Catalog # 11067-GP) is immobilized at 0.5 µg/mL (100 µL/well) Recombinant Human IL-6 (Catalog # 7270-IL) in the presence of Recombinant Human IL-6R alpha (Catalog # 227-SR) binds with an ED50 of 0.200-3.00 ng/mL.

Scientific Data Images for Recombinant Human gp130 His-tag Protein, CF

Recombinant Human gp130 His-tag Protein Binding Activity.

When Recombinant Human gp130 His-tag Protein (Catalog # 11067-GP) is immobilized at 0.5 µg/mL (100 µL/well) Recombinant Human IL-6 (7270-IL) in the presence of Recombinant Human IL-6R alpha (227-SR) binds with an ED50 of 0.200-3.00 ng/mL.

Recombinant Human gp130 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human gp130 His-tag Protein (Catalog # 11067-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-100 kDa.

Formulation, Preparation and Storage

11067-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Human gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 597 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 277 aa cytoplasmic tail. The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (1-2). The ECD of human gp130 shares 73% and 75% aa sequence identity with that of mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)‑6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2). 

References

  1. Wang, Y. et al. (1992) Genomics 14:666.
  2. Muller-Newen, G. (2003) Sci. STKE pe40.
  3. Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
  4. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
  5. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.

Long Name

Glycoprotein 130

Alternate Names

CD130, IL6ST

Entrez Gene IDs

3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)

Gene Symbol

IL6ST

UniProt

Additional gp130 Products

Product Documents for Recombinant Human gp130 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human gp130 His-tag Protein, CF

For research use only

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